Features of trans-arterial embolization with large microspheres of rats and rabbits

Studying of large microspheres (MS) from polyvinyl alcohol with the diameter of (d) of 0.2-0.6 mm in the infusional volume of 0.4-0.9 ml is directed for detection of their features necessary for preclinical study with surgical access to a femoral artery. Two problems were solved: development of trans-arterial (t.a.) technique introductions to rats and efficiency assessment t.a. embolization on rabbits. Experiments on the rats under general anesthesia are made with d=0.2-0.3 mm of MS. For preclinical study of the technique on rats with the body weight of 120-150 g, infusional system with catheter of G26 and period of observation within 7 days allowing to control efficiency of embolization of tumor intertwined in the blood supply zone is recommended. Experiments on rabbits under general anesthesia are made with MS diameter of 0.4-0.6 mm of MS. By means of X-ray contrast visualization decrease in intensity of a regional blood-stream is shown expressed up to full and long (not less than 14 days) for all of the volumes of MS. The best results of embolization are received with G20 catheter in infusion system. Local trophic changes under the influence of MS (side effects) are regarded as modeling artifact on rabbits connected with full switch off of blood-groove in rather small vessel and violation of innervation of the extremity. The analysis of the revealed features with selection of adequate characteristics of the ISS and tools reached efficiency and complications of trans-arterial embolization allows to recommend the fulfilled technique on rats for preclinical study of large ISS from polyvinyl alcohol, and to consider LMS perspective for trans-arterial embolization or chemoembolization of tumor.

large microspheres, technique, rats, rabbits, trans-arterial embolization, blood stream, side effects