Phagocytic and chemiluminescent activity of blood neutrophils in patients with bladder cancer

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Abstract

BACKGROUND: Tumor microenvironment modulates (including with the help of metabolites) the functional activity of the neutrophils that contribute to the reprogramming of the antitumor activity into a protumor one.

AIMS: To study the phagocytic and chemiluminescent activity of neutrophils in patients with bladder cancer (BC) under the influence of metabolites of the tumor microenvironment in vitro.

MATERIALS AND METHODS: We examined 37 patients with superficial BC (T1,а,isN0M0) and considered 32 healthy individuals as a control group. Neutrophils isolated from their blood were incubated in vitro with lactate, ADP, and glutamate. Phagocytic activity was examined using flow cytometry, and the intensity of the respiratory burst of neutrophils was evaluated via chemiluminescent analysis.

RESULTS: In patients with BC, the phagocytic index (PhI) values are reduced compared to the control sample (without in vitro metabolite exposure) and when exposed to glutamate, while the effect of lactate on cells causes an increase in the phagocytic number and PhI. Moreover, under the influence of lactate in vitro, the activity of spontaneous and zymosan-induced chemiluminescence of neutrophils decreases. ADP causes a decrease in spontaneous chemiluminescence only. Finally, under the influence of glutamate, the indicators of spontaneous and induced chemiluminescence decrease.

CONCLUSIONS: Under the influence of lactate and ADP (products of tumor cells), the phagocytic activity of a population of immature neutrophils is stimulated, which leads to myeloid suppressor cells that inhibit antitumor immunity. Thus, metabolites of the tumor microenvironment modulate the activity of the respiratory burst of neutrophils in patients with BC.

About the authors

Andrei A. Savchenko

Krasnoyarsk Scientific Center of the Siberian Branch of the Russian Academy of Sciences, Separate Division of the Research Institute of Medical Problems of the North; Krasnoyarsk State Medical University named after Prof. V.F. Voino-Yasenetsky

Email: aasavchenko@yandex.ru
ORCID iD: 0000-0001-5829-672X
SPIN-code: 3132-8260

MD, Dr. Sci. (Med.), Professor

Russian Federation, Krasnoyarsk; Krasnoyarsk

Ruslan A. Zukov

Professor V.F. Voino-Yasenetsky Krasnoyarsk State Medical University; Krasnoyarsk Regional Clinical Oncology Dispensary named after A.I.Kryzhanovsky RSBHE

Email: ethics.commitee@mail.ru
ORCID iD: 0000-0002-7210-3020
SPIN-code: 3632-8415

MD, Dr. Sci. (Med.), Professor, Head Physician of A.I. Kryzhanovsky Krasnoyarsk Regional Clinical Oncology Center

Russian Federation, Krasnoyarsk; Krasnoyarsk

Michael A. Firsov

Professor V.F. Voyno-Yasenetsky Krasnoyarsk State Medical University; Krasnoyarsk Clinical Regional Hospital

Email: firsma@mail.ru
ORCID iD: 0000-0002-0887-0081
SPIN-code: 6308-6260

Cand. Sci. (Med.), Head of the Department of Urology, Andrology and Sexology of the Institute of Postgraduate Education

 

Russian Federation, 1, Partizana Zheleznyaka St., Krasnoyarsk, 660022; 3A, Partizana Zheleznyaka St., Krasnoyarsk, 660022

Evgeniy V. Slepov

Krasnoyarsk Scientific Center of the Siberian Branch of the Russian Academy of Sciences, Separate Division of the Research Institute of Medical Problems of the North; Krasnoyarsk Regional Clinical Oncology Dispensary named after A.I.Kryzhanovsky RSBHE

Email: slepov99@mail.ru
ORCID iD: 0000-0002-3787-3126
SPIN-code: 2097-0304

MD, Cand. Sci. (Biol.)

Russian Federation, Krasnoyarsk; Krasnoyarsk

Vasiliy D. Belenyuk

Krasnoyarsk Scientific Center of the Siberian Branch of the Russian Academy of Sciences, Separate Division of the Research Institute of Medical Problems of the North

Email: dyh.88@mail.ru
ORCID iD: 0000-0003-2848-0846
SPIN-code: 6195-6630
Russian Federation, Krasnoyarsk

Ivan I. Gvozdev

Krasnoyarsk Scientific Center of the Siberian Branch of the Russian Academy of Sciences, Separate Division of the Research Institute of Medical Problems of the North

Email: leshman-mult@mail.ru
ORCID iD: 0000-0002-1041-9871
SPIN-code: 6203-4651

младший научный сотрудник лаборатории НИИ медицинских проблем Севера

Russian Federation, Krasnoyarsk

Alexander G. Borisov

Krasnoyarsk Regional Clinical Oncology Dispensary named after A.I.Kryzhanovsky RSBHE; Professor V.F. Voino-Yasenetsky Krasnoyarsk State Medical University

Author for correspondence.
Email: 2410454@mail.ru
ORCID iD: 0000-0002-9026-2615
SPIN-code: 9570-2254

MD, Cand. Sci (Med.)

Russian Federation, Krasnoyarsk; Krasnoyarsk

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Copyright (c) 2021 Savchenko A.A., Zukov R.A., Firsov M.A., Slepov E.V., Belenyuk V.D., Gvozdev I.I., Borisov A.G.

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
 


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