CHANGES IN PHAGOCYTOSIS PARAMETERS WITH A MARKER OF PHAGOSOME ACIDIFICATION pHrodo™ UNDER-ZYMOSAN-INDUCED PERITONITIS IN RATS

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The changes in phagocytosis under 12-hour zymosan-induced peritonitis in rats were investigated using flow laser cytometry using zymosan labeled with pH-sensitive flurochrome green pHrodo™ (Thermo Fisher Scientifi c). An increase in the absolute parameters of phagocytosis was found in the study of peritoneal exudate cells, blood leukocytes and bone marrow cells in rats with peritonitis in comparison with intact animals.

作者简介

S. Shilov

“Institute of Ecology and Genetics of Microorganisms of the Ural Branch of the Russian Academy of Sciences” – a branch of the PFRC UB RAS;
Acad. E. A. Wagner Perm State Medical University

编辑信件的主要联系方式.
Email: stshilov@mail.ru

PhD, MD, Junior Researcher,

Perm

俄罗斯联邦

Ju. Shilov

“Institute of Ecology and Genetics of Microorganisms of the Ural Branch of the Russian Academy of Sciences” – a branch of the PFRC UB RAS;
Acad. E. A. Wagner Perm State Medical University

Email: fake@neicon.ru

PhD, MD, Associate Professor, Leading Researcher,

Perm

俄罗斯联邦

Ya. Tulyaev

“Institute of Ecology and Genetics of Microorganisms of the Ural Branch of the Russian Academy of Sciences” – a branch of the PFRC UB RAS

Email: fake@neicon.ru

Aspirant,

Perm

俄罗斯联邦

S. Barkov

Acad. E. A. Wagner Perm State Medical University

Email: fake@neicon.ru

Student, 

Perm

俄罗斯联邦

参考

  1. Zhang R., Kobayashi Y., Kazumura K., Tsuchiya H., Morishita N., Inagawa H., Soma G. Development of an evaluation device for phagocytic activity of new phagocytes using simple and pH-sensitive particles that do not require pre-treatment. Anticancer Res. 2016, 36(7), 3613–3618.
  2. Kapellos T. S., Taylor L., Lee H., Cowley S. A., James W. S.., Iqbal A. J., Greaves D. R. A novel real time imaging platform to quantify macrophage phagocytosis. Biochemical Pharmacology 2016, 116, 107–119.
  3. Шилов Ю. И., Шилов С. Ю., Жукова А. Е., Барков С. Ю., Петухова А. А. Изменение уровня ацидификации фагосом у крыс в зависимости от фазы эстрального цикла. Российский иммунологический журнал 2016, том 10 (19), № 2 (1), 183–184.

版权所有 © Shilov S.J., Shilov J.I., Tulyaev Y.A., Barkov S.Y., 2019

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此作品已接受知识共享署名 4.0国际许可协议的许可
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