EFFECT OF TRANSPLANTATION OF MESENCHIMAL STEM CELLS (MSC) AND PRODUCED MICROVESICLES (MV) ON THE MAINTENANCE OF MEMORY T-CELLS AND T-REGULATORY CELLS IN THE MODEL ACUTE RENAL FAILURE IN MICE

MV has been shown to have a positive effect on the restoration of renal excretory function at a level comparable to the effect of MSCs. The use of MSC and MB in the treatment of acute renal failure resulted in almost the same result: on the 4th day the level of urea was 9.3 μg/ml and 9.37 μg/ml, respectively, which is 25% lower than in the glycerol control. By the 8th day, the level of urea was 7.9 μg/ ml after the introduction of MSC and 7.6 μg/ml after the introduction of MB, which is 21% lower than in the glycerol control. It has been shown that the MW produced by MSCs stimulate the proliferation of both splenocytes and cells of the kidney tissue in a dose-dependent manner. To study the resistance to apoptosis, splenocytes were used both from intact mice and from glycerol-induced ARF. The addition of MB causes a decrease in the level of apoptosis in both intact mice (21.73%) and mice with ARF (13.28%). Accordingly, inversely, the percentage of cells in the S / M phase of the cell cycle increased.

mesenchymal stem cells (MSC), microvesicles (MV), acute renal failure (ARF)