DENDRITIC CELLS ELECTROPORATION WITH DNA-PLASMID ENCODING IL-10

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Dendritic cells (DC) are widely used to produce vaccines for the treatment of various types of diseases, such as malignant neoplasms, autoimmune disorders, rejection and GVHD. To control the functional properties of DCs, they are transfected with various immunoregulatory proteins, including IL-10. In this paper, the optimization of the method of electrical transfection (electroporation) of DCs with a DNA plasmid encoding IL-10 was performed. The effi ciency of DC electroporation (EP) reaches 45‒72% with cell death below 7%. The active expression of IL-10 in transfected DC was shown within 3 days after EP. According to the results of the work, EP was recognized as an eff ective method for obtaining transfected DCs for the production of cellular vaccines.

作者简介

V. Tereshchenko

FSBSI Research institute of fundamental and clinical immunology

编辑信件的主要联系方式.
Email: tervp@ngs.ru

Ph.D studentin laboratory of molecular immunology,

Novosibirsk

俄罗斯联邦

J. Khantakova

FSBSI Research institute of fundamental and clinical immunology

Email: fake@neicon.ru

Ph.D, researcherin laboratory of molecular immunology,

Novosibirsk

俄罗斯联邦

V. Kurilin

FSBSI Research institute of fundamental and clinical immunology

Email: fake@neicon.ru

Ph.D, researcherin laboratory of molecular immunology,

Novosibirsk

俄罗斯联邦

A. Silkov

FSBSI Research institute of fundamental and clinical immunology

Email: fake@neicon.ru

Ph.D, researcherin laboratory of molecular immunology, head of RIFCI,

Novosibirsk

俄罗斯联邦

R. Maksyutov

SRC of Virology and Biotechnology “Vector”

Email: fake@neicon.ru

Ph.D, researcher,

Koltsovo

俄罗斯联邦

参考

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版权所有 © Tereshchenko V.P., Khantakova J.N., Kurilin V.V., Silkov A.N., Maksyutov R.A., 2019

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