EFFECT OF MACROPHAGE CONDITION MEDIA ON PHENOTYPE AND PROLIFERATION OF HUMAN NEUROBLASTOMA CELL LINE SH-SY5Y

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The study investigated the effect of conditioned media of M1 and M2 macrophages on the phenotype and proliferation of the human neuroblastoma cell line SH-SY5Y. It has been established that conditioned media of M2 macrophages, generated in the presence of GM-CSF under defi ciency of growth factors, have a pronounced stimulating effect on the SH-SY5Y cell proliferation. The expression of the low-affinity nerve growth factor receptor, CD271, correlates with cell morphology: in cultures with a predominance of N-type cells, the number of CD271+ cells is signifi cantly higher compared to cultures dominated by S-type cells.

作者简介

I. Rashchupkin

Federal Budget Institution of Science «Research Institute of Fundamental and Clinical Immunology»

编辑信件的主要联系方式.
Email: iwwwanbets@mail.ru

second year residentof the Laboratory of Cellular Immunotherapy,

Novosibirsk

俄罗斯联邦

M. Tikhonova

Federal Budget Institution of Science «Research Institute of Fundamental and Clinical Immunology»

Email: fake@neicon.ru

PhD (Biology), Senior Research Associate, Laboratory of Cellular Immunotherapy,

Novosibirsk

俄罗斯联邦

T. Tyrinova

Federal Budget Institution of Science «Research Institute of Fundamental and Clinical Immunology»

Email: fake@neicon.ru

PhD (Medicine), Research Associate, Laboratory of Cellular Immunotherapy,

Novosibirsk

俄罗斯联邦

L. Sakhno

Federal Budget Institution of Science «Research Institute of Fundamental and Clinical Immunology»

Email: fake@neicon.ru

PhD (Biology), Senior Research Associate, Laboratory of Cellular Immunotherapy,

Novosibirsk

俄罗斯联邦

E. Shevela

Federal Budget Institution of Science «Research Institute of Fundamental and Clinical Immunology»

Email: fake@neicon.ru

PhD, MD (Medicine), Leading Research Associate, Laboratory of Cellular Immunotherapy, 

Novosibirsk

俄罗斯联邦

参考

  1. Hu X., Leak R.K., Shi J., Suenaga J., Gao Y., Zheng P., Chen J. Microglial and macrophage polarization – new prospects for brain repair. Nat Rev Neurol. 2015,11(1), 56–64.
  2. Sakhno L.V., Shevela E.Ya., Tikhonova M. A., Ostanin A. A, Chernykh E. R. Scandinavian journal of Immunology. 2015. 83, 151–159.
  3. Ferlemann F. C., Menon V., Condurat A. L., Rößler J., Pruszak J. Surface marker profiling of SH-SY5Y cells enables small molecule screens identifying BMP4 as a modulator of neuroblastoma differentiation. Sci Rep. 2017, 7(1), 1–14.

版权所有 © Rashchupkin I.M., Tikhonova M.A., Tyrinova T.V., Sakhno L.V., Shevela E.Y., 2019

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