DIFFERENTIATED APPROACH IN EVALUATING THE RESPONSE OF IMMUNE SYSTEM TO THE PRESENCE OF M.TUBERCULOSIS WITH DIFFERENT DRUG SUSCEPTIBILITY

The purpose of the study has a comparative study of quantitative and functional state of the immune system cells in patients with infiltrative pulmonary tuberculosis, which is caused by drug-sensitive and drug-resistant disease. The study included 29 patients, of which: from 12 people the disease was caused by drug-sensitive M.tuberculosis, 17 - the causative agent had multidrug resistance and 25 healthy people. We estimated the number of T- (CD45⁺CD3⁺) and B-lymphocytes (CD45⁺CD19⁺), NK-cells (CD45⁺CD3CD16⁺CD56⁺), T-helper cells (CD3⁺CD4⁺), cytotoxic T cells (CD3⁺CD8⁺), γδ-T-lymphocytes (CD3^brightCD4^-), NKT-cells (CD3⁺CD16⁺CD56⁺), activated T-lymphocytes and by the expression of T-helper cells of CD25 and HLA-DR, the probability of apoptosis of CD95 expression, the number of T-reg-cells (CD3⁺CD4⁺CD127CD25⁺) by flow cytometry on Coulter^®Epics^®XL instrument (Beckman Coulter, USA). Statistical data analysis was performed using Microsoft software, USA (Office Excel 2007) and StatSoft, USA (Statistica For Windows v.6.1.). Established that infiltrative tuberculosis caused by drug-sensitive M.tuberculosis, accompanied by an increase in HLA-DR expression and decrease in CD95 on T-lymphocytes, reducing the amount of B-cells, increased number of NK-cells. Infiltrative tuberculosis caused by M.tuberculosis multidrug resistance, is characterized by the absence of reducing CD95 on T-lymphocytes, a decrease in the population of NKT cells. Immunological criterion for evaluating drug sensitivity M.tuberculosis is the absolute quantity of CD3⁺HLA^-DR⁺-cells.

NKT-клетки, γδ-T-лимфоциты, СD3⁺HLA-DR⁺, В-лимфоциты, туберкулез, NKT-cells, γδ-T-lymphocytes, СD3⁺HLA-DR⁺, В-lymphocytes, tuberculosis