Content of RANTES, IL-8 and MIP-1β chemokines in platelet lysates obtained from donor blood

Growth supplements obtained from animal sera have a rather variable range of a specific growth factor contents and are subject to potential contamination. Moreover, the growth supplements obtained from animal raw materials contain a composition of proteins, vitamins and microelements necessary for full-fledged cell growth, and one may overcome these drawbacks by replacing them with a product obtained from human donor blood. Platelet lysate contains biologically active molecules derived from endogenous granules of these blood elements. Concentration of distinct biocomponents in the resulting product is variable, depending not only on gender and blood group, but also on other, as yet unidentified individual characteristics. The aim of this study was to examine the content of some chemokines, in particular, RANTES, MIP-1β and IL-8, in platelet lysates obtained from healthy donors. The highest concentrations of MIP-1β cytokine were noted in Rh-negative donors, in females with blood group III, and in males with the IV. blood group. The highest IL-8 contents in platelet lysate were also recorded in Rh-negative, group IV men and women. Platelet lysate of volunteers with the II blood group (men, Rh-negative; women, Rh-positive) contained the highest amount of RANTES. Significant differences were found between the MIP-1β levels in thrombolysate of men and women with the second A (II), and AB (IV) Rh-negative blood groups; between women of the third B (III) blood group with different Rh factors. Moreover, statistically significant differences were found in the RANTES/CCL5 chemokine values in the thrombolysate for the groups of men and women with the second A (II) [with R (+) and R (-)], and AB (IV) blood group (Rh+). Interestingly, in Rh (-) men with the first 0 (I) and second A (II) blood groups, the content of chemokine RANTES/CCL5 in the blood thrombolysate significantly exceeded similar values in the blood obtained from Rh-positive men. Conclusion: The relationship between the donors’ belonging to a particular blood group and the content of these chemokines is not yet clear. Future studies with deep bioinformatics analysis will reveal the unclear interrelations between these indicators.