Effect of Phlojodicarpus sibiricus on the subpopulation profile of blood cells and thymus of mice with a simulated tumor
- Authors: Egorov A.N.1, Pashkina E.A.2, Golderova A.S.1
-
Affiliations:
- Ammosov North-Eastern Federal University
- Research Institute of Fundamental and Clinical Immunology
- Issue: Vol 28, No 3 (2025)
- Pages: 509-514
- Section: SHORT COMMUNICATIONS
- URL: https://journals.rcsi.science/1028-7221/article/view/319893
- DOI: https://doi.org/10.46235/1028-7221-17182-EOP
- ID: 319893
Cite item
Full Text
Abstract
The present study concerned the effect of Phlojodicarpus sibiricus extract on immune response in melanoma model induced by the B16 cells in C57Black/6 mice. The aim of our work was to evaluate the immunomodulatory properties of this plant extract in the context of oncogenesis. To achieve this goal, an experiment was conducted with three groups of mice: a control group of healthy animals, a group with induced melanoma receiving the extract, and a non-treated melanoma group. Melanoma malignancy was modeled by injecting a suspension of B16 cells, and the extract of Phlojodicarpus sibiricus was administered orally after the first signs of developing melanoma. Changes in lymphocyte subpopulation profile of blood and thymus were assessed using flow cytometry with specific monoclonal antibody markers for T lymphocytes, T helper cells, T killer cells, B lymphocytes, NK cells, and T regulatory cells. The results obtained did not reach statistical significance (p < 0.05). However, certain trends were observed in the thymus, where a decreased content of T regulatory cells and NK cells was noted in the group receiving the extract compared to the melanoma group. This reduction may indicate a potential influence of the extract on the antitumor immune response, whereas changes in the ratio of T helper/T killer cells suggest possible regulation of cellular immunity. The reduction in B lymphocyte levels may reflect a shift in immune response from humoral to cell-mediated pattern, which could be beneficial in the context of tumor pathology. The extract of Phlojodicarpus sibiricus appears to modulate melanoma development, suggesting a potential for further research in immunomodulation and anti-tumor therapy. The study has revealed a reduced level of T regulatory cells and NK cells in the extract group, thus suggesting a diminished antitumor immune response. Moreover, a decline in B lymphocytes suggests a shift from humoral to cell-mediated immunity. These findings highlight the potential activity of extract as an immunomodulating agent. However, further research is necessary to confirm these effects. Mechanistic analysis of the extract action would be crucial, particularly, the ways of lymphocyte activation and apoptosis regulation.
Keywords
Full Text
##article.viewOnOriginalSite##About the authors
A. N. Egorov
Ammosov North-Eastern Federal University
Email: 291219942014@mail.ru
Postgraduate Student at the Institute of Natural Sciences
Russian Federation, YakutskE. A. Pashkina
Research Institute of Fundamental and Clinical Immunology
Email: 291219942014@mail.ru
PhD (Biology), Head of the Laboratory of Immune Response Regulation, Associate Professor at the Department of Clinical Immunology
Russian Federation, NovosibirskA. S. Golderova
Ammosov North-Eastern Federal University
Author for correspondence.
Email: 291219942014@mail.ru
PhD, MD (Medicine), Professor at the Department of Health Organization and Preventive Medicine of the Medical Institute
Russian Federation, YakutskReferences
- Georgievsky V.P. Biologically Active Substances of Medicinal Plants. Novosibirsk: Nauka. Sibirskoye otdeleniye, 1990. 336 p.
- Egorov A.N., Tikhonov D.G., Golderova A.S. Antiproliferative Effect of wild berries and Phlojodicarpus sibiricus on MCF-7 breast cancer cells from Northeast Siberia. Uspekhi molekulyarnoy onkologii = Advances in Molecular Oncology, 2024, Vol. 11, no. 3, pp. 126-132. (In Russ.)
- Red Data Book of the Republic of Sakha (Yakutia). Vol. 1. Rare and Endangered Species of Plants and Fungi. Moscow: Reart, 2017. 420 p.
- Samoilova G.S., Goryachko M.D., Diakonov V.M., Goryachko M.D., Prokinova A.N., Pavlinov P.S. Sakha (Yakutia). In: Great Russian Encyclopedia [Electronic resource]. Updating: GRE editors, 2020. Available at: https://web.archive.org/web/20221206175520/https://bigenc.ru/geography/text/5753444.
- Khandy M.T., Kochkin D.V., Tomilova S.V., Galishev B.A., Klyushin A.G., Nosov A.M. Obtainment and Phytochemical Screening of Callus and Suspension Cell Cultures of Phlojodicarpus sibiricus (Steph. ex Spreng.) К.-Pol. Biotekhnologiya = Biotekhnologiya, 2020, Vol. 36, no. 5, pp. 54-61. (In Russ.)
- Shurigin A.Ya., Aseeva T.A., Skorokhod N.S., Abramova N.O., Gerasimenko Yu.G. Influence on Neurite Growth of Spinal Ganglia of Chicken Embryos by Ethanol Extract of Phlojodicarpus sibiricus. Uspekhi sovremennogo yestestvoznaniya = Achievements in Modern Natural Science, 2005, no. 10, pp. 90-91. (In Russ.)
- Durgeau A., Virk Y., Corgnac S., Mami-Chouaib F. Recent advances in targeting CD8 T-cell immunity for more effective cancer immunotherapy. Front. Immunol., 2018, Vol. 9, 14. doi: 10.3389/fimmu.2018.00014.
- Mahmoudi Z., Soleimani M., Saidi A., Khamisipour G., Azizsoltani A. Effects of Foeniculum vulgare ethanol extract on osteogenesis in human mesenchymal stem cells. Avicenna J. Phytomed., 2013, Vol. 3, pp. 135-142.
- Minetto P., Guolo F., Pesce S., Greppi M., Obino V., Ferretti E., Sivori S., Genova C., Lemoli R.M., Marcenaro E. Harnessing NK cells for cancer treatment. Front. Immunol., 2019, Vol. 10, 2836. doi: 10.3389/fimmu.2019.02836.
- Olennikov D.N., Fedorov I.A., Kashchenko N.I., Chirikova N.K., Vennos C. Khellactone derivatives and other phenolics of Phlojodicarpus sibiricus (Apiaceae): HPLC-DAD-ESI-QQQ-MS/MS and HPLC-UV profile, and antiobesity potential of dihydrosamidin. Molecules, 2019, 24,Vol. 12, 2286. doi: 10.3390/molecules24122286.
- Shevach E.M. CD4+CD25+ Suppressor T cells: more questions than answers. Nat. Rev. Immunol., 2002, Vol. 2, no. 6, pp. 389-400.
- Valdman A., Jaraj S.J., Comperat E., Charlotte F., Roupret M., Pisa P., Egevad L. Distribution of Foxp3-, CD4-, and CD8-positive lymphocytic cells in benign and malignant prostate tissue. APMIS, 2010, Vol. 118, pp. 360-365.
- Zha X., Xu Z., Liu Y., Xu L., Huang H., Zhang J., Cui L., Zhou C., Xu D. Amentoflavone enhances osteogenesis of human mesenchymal stem cells through JNK and p38 MAPK pathways. J. Nat. Med., 2016, Vol. 70, pp. 634-644.
- Zhang H., Liu L., Zhang J., Chen J., Ye J., Shukla S., Qiao J., Zhan X., Chen H., Wu C.J., Fu Y.-X., Li B. Investigation of antigen-specific T-cell receptor clusters in human cancers. Clin. Cancer Res., 2019, Vol. 26, 3249. doi: 10.1158/1078-0432.CCR-19-3249.
Supplementary files
