Polymorphisms of cytokine genes and their correlations with efficiency of continuous treatment in patients with pulmonary tuberculosis
- Authors: Alymenko M.A.1,2, Valiev R.S.2, Valiev N.R.2, Balabanova N.P.1, Kolchanova N.E.3, Polybin R.V.4, Shepel R.N.5, Lipatov V.A.6, Kolomiets V.M.6
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Affiliations:
- Synergy University
- Kazan State Medical Academy, a Branch of the Russian Medical Academy of Continuous Postdegree Education
- Gomel State Medical University
- I. Sechenov First Moscow State Medical University
- National Medical Research Center of Therapy and Preventive Medicine
- Kursk State Medical University
- Issue: Vol 28, No 2 (2025)
- Pages: 279-286
- Section: SHORT COMMUNICATIONS
- URL: https://journals.rcsi.science/1028-7221/article/view/284861
- DOI: https://doi.org/10.46235/1028-7221-17016-POC
- ID: 284861
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Abstract
Current research in the field of an immunogenetics of tuberculosis is considered a key direction, since the treatment efficiency and outcomes of the disease in most cases depend on immunological and genetic features of the patient. Special attention should be paid to IL-10 value in development of protective immunity against tuberculosis. There are some contradictory data on influence of IL-10 on development of immune response in the patients with pulmonary tuberculosis which are published in several works. For example, L.G. Tarasova and colleagues connect the increased concentration of IL-10 with extensive destructive processes in pulmonary tissue, whereas while D. Higgins and coauthors show its key role in protection against chronic pneumonia. The objective of our study was to evaluate relations between several cytokine gene polymorphisms (IL1β, IL4, IL10, TNF), and their expression levels of in the course of continuous chemotherapy.
Whole venous blood was taken for the molecular and genetic analysis was perfoemed, with subsequent isolation of genomic DNA and carrying out real-time PCR for genotyping of SNPs. Concentration of cytokines in blood serum were defined by method of enzyme immunoassay. Statistical analysis included check of normality of distribution of data, nonparametric correlations and comparison of qualitative characters. The assessment of compliance of genotypes to distribution of Hardy–Weinberg was carried out with use of criterion χ2 Pearson. To measure the cytokine concentrations (IL-1β, IL-4, IL-6, TNFα, IFNγ, IL-10) in blood serum, we used peripheral venous blood taken in fasting state at a 5-mL volume. Enzyme immunoassay was made with reagent sets from JSC Vektor Best-Tsitokiny according to the instructions from manufacturer.
In the course of specific chemotherapy at the continuation phase, a more adverse course of a disease was registered among the patients with a pulmonary tuberculosis harboring IL4-589CC genotype, when compared with those who have IL4-589CT+TT genotype. In the persons with IL10-592CA+AA genotype, a less favorable course of the disease was observed at the intensive phase of therapy, in comparison with patients who had IL10-592CC genotype.
Thus, process of interaction between microorganisms and macroorganisms in case of a tuberculosis infection represents a quite complicated process involving a set of immune response elements. Interactions within this system are controlled by means of cytokines, the mediators of cellular interactions.
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##article.viewOnOriginalSite##About the authors
M. A. Alymenko
Synergy University; Kazan State Medical Academy, a Branch of the Russian Medical Academy of Continuous Postdegree Education
Author for correspondence.
Email: maxim.alymenko@gmail.com
PhD (Medicine), Associate Professor, Department of General Biology and Pharmacy; Assistant Professor, Department of Phthisiology and Pulmonology
Russian Federation, Moscow; KazanR. Sh. Valiev
Kazan State Medical Academy, a Branch of the Russian Medical Academy of Continuous Postdegree Education
Email: maxim.alymenko@gmail.com
PhD, MD (Medicine), Professor, Chief Phthisiatrician of the Volga Federal District, Honored Doctor of Russia and the Republic of Tatarstan, Head, Department of Phthisiology and Pulmonology
Russian Federation, KazanN. R. Valiev
Kazan State Medical Academy, a Branch of the Russian Medical Academy of Continuous Postdegree Education
Email: maxim.alymenko@gmail.com
PhD (Medicine), Associate Professor, Department of Phthisiology and Pulmonology
Russian Federation, KazanN. P. Balabanova
Synergy University
Email: maxim.alymenko@gmail.com
PhD (Biology), Associate Professor, Head, Department of General Pharmacology and Pharmacy
Russian Federation, MoscowN. E. Kolchanova
Gomel State Medical University
Email: maxim.alymenko@gmail.com
PhD (Medicine), Associate Professor, Department of Microbiology, Virology and Immunology
Belarus, GomelR. V. Polybin
I. Sechenov First Moscow State Medical University
Email: maxim.alymenko@gmail.com
PhD (Medicine), Associate Professor, Chief Non-Staff Specialist Epidemiologist of the Russian Ministry of Health, Deputy Director for Scientific Work of F. Erisman Institute of Public Health, Associate Professor, Department of Epidemiology and Evidential Medicine
Russian Federation, MoscowR. N. Shepel
National Medical Research Center of Therapy and Preventive Medicine
Email: maxim.alymenko@gmail.com
PhD (Medicine), Associate Professor, Chief Non-Staff Specialist Therapist of the CFD of the Russian Ministry of Health, Leading Research Associate, Deputy Director for Perspective Development of Medical Activity, Head, Department of Scientific and Strategic Development of Primary Health Care, Associate Professor, Department of Public Health Care and Organization of Health Care
Russian Federation, MoscowV. A. Lipatov
Kursk State Medical University
Email: maxim.alymenko@gmail.com
Vice-Rector for Scientific Work and Innovative Development, Professor of the Department of Operative Surgery and Topographic Anatomy, Head of the Laboratory of Experimental Surgery and Oncology, Research Institute of Experimental Medicine
Russian Federation, KurskV. M. Kolomiets
Kursk State Medical University
Email: maxim.alymenko@gmail.com
Professor, Department of Clinical Immunology, Allergology and Phthisiopulmonology
Russian Federation, KurskReferences
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