Hormone-producing role of mast cells in reproductive processes: myth or reality?
- Authors: Valikova O.V.1,2,3, Zdor V.V.1,4, Tikhonov Y.N.1, Boroda A.V.5
-
Affiliations:
- Pacific State Medical University
- Regional Clinical Hospital No. 2
- Plastek Surgery Cliniс
- Cliniс for Diabetes and Endocrine Diseases
- A. Zhirmunsky National Scientific Center of Marine Biology of the Far East Branch of the Russian Academy of Sciences
- Issue: Vol 28, No 2 (2025)
- Pages: 189-194
- Section: SHORT COMMUNICATIONS
- URL: https://journals.rcsi.science/1028-7221/article/view/284813
- DOI: https://doi.org/10.46235/1028-7221-17043-HPR
- ID: 284813
Cite item
Full Text
Abstract
The role of mast cells in the onset and progression of endocrine system dysfunction has been studied for quite a long time, but a clear understanding of their participation has not been obtained. The purpose of the study was to consider the hormone-producing and cytokine-producing functions of mast cells in primary culture in various environments simulating certain hormonal conditions, modeling the level of hormones in follicular fluid during ovulation, to identify the participation of mast cells in the development of endocrine diseases, and to discover new functions of mast cells.
A continuous line of human mast cells HMC-1 (Human mast cell, ATCC) was used, being cultured in IMDM medium (Dulbecco’s modified Eagle’s medium, Sigma-Aldrich). Medium 1 (IMDM) served as a control; medium 2 was an IMDM medium supplied with hormones simulating follicular fluid during ovulation in healthy women, and medium 3 (IMDM) was supplied with hormones mimicking follicular fluid during ovulation in polycystic ovary syndrome. The content of progesterone, testosterone, estradiol, IL-6 was measured on days 1,3,7 of experimental cultures.
Hormonal pattern in the mast cell cultures changed depending on the microenvironment and duration of experiment. The level of estradiol in medium 1 increased more than 80 times compared to the level of estradiol before initiation the cultures. Incubation in medium 2 was followed by 40-fold increase in estradiol by the 7th day of experiment. In medium 3, estradiol levels increased more than 35 times. Testosterone level increased in medium 1 by 3 times; in medium 2, 2.5-fold by the day 7. Upon culture in medium 3, testosterone levels increased 8-fold. IL-6 levels in all media with mast cell culture increased by day 7, but did not exceed the physiological ranges of the cytokine.
A significant progressive increase in the contents of estradiol, testosterone and IL-6 in continuous culture of HMC-1 mast cells observed in different culture media simulating certain in vivo hormonal background suggests an active participation of mast cells in reproductive processes and an independent de novo synthesis of sex steroids and IL-6 by mast cells, which, however, requires a number of confirmatory experiments.
Keywords
Full Text
##article.viewOnOriginalSite##About the authors
O. V. Valikova
Pacific State Medical University; Regional Clinical Hospital No. 2; Plastek Surgery Cliniс
Author for correspondence.
Email: renalex.99@mail.ru
Junior Researcher; Clinical Endocrinologist
Russian Federation, Vladivostok; Vladivostok; VladivostokV. V. Zdor
Pacific State Medical University; Cliniс for Diabetes and Endocrine Diseases
Email: renalex.99@mail.ru
PhD, MD (Medicine), Leading Researcher; Clinical Endocrinologist
Russian Federation, Vladivostok; VladivostokYa. N. Tikhonov
Pacific State Medical University
Email: renalex.99@mail.ru
Senior Lecturer, Department of Pathological Anatomy
Russian Federation, VladivostokA. V. Boroda
A. Zhirmunsky National Scientific Center of Marine Biology of the Far East Branch of the Russian Academy of Sciences
Email: renalex.99@mail.ru
PhD (Biology), Senior Researcher, Deputy Director for Research
Russian Federation, VladivostokReferences
- Borthakur A., D Prabhu Y., Valsala Gopalakrishnan A. Role of IL-6 signalling in Polycystic Ovarian Syndrome associated inflammation. J. Reprod. Immunol., 2020, Vol. 141, 103155. doi: 10.1016/j.jri.2020.103155.
- da Silva E.Z., Jamur M.C., Oliver C. Mast cell function: a new vision of an old cell. J. Histochem. Cytochem., 2014, Vol. 62, no. 10, pp. 698-738.
- Frungieri M.B., Calandra R.S., Mayerhofer A., Matzkin M.E. Cyclooxygenase and prostaglandins in somatic cell populations of the testis. Reproduction, 2015, Vol. 149, no. 4, pp. 169-180.
- Guhl S., Artuc M., Zuberbier T., Babina M. Testosterone exerts selective anti-inflammatory effects on human skin mast cells in a cell subset dependent manner. Exp. Dermatol., 2012, Vol. 21, no. 11, pp. 878-880.
- Gunin A.G., Sharov A.A. Role of mast cells in oestradiol effects on the uterus of ovariectomized rats. J. Reprod. Fertil., 1998, Vol. 113, no. 1, pp. 61-68.
- Jensen F., Woudwyk M., Teles A., Woidacki K., Taran F., Costa S., Malfertheiner S.F., Zenclussen A.C. Estradiol and progesterone regulate the migration of mast cells from the periphery to the uterus and induce their maturation and degranulation. PLoS One, 2010, Vol. 5, no. 12, 14409. doi: 10.1371/journal.pone.0014409.
- McCallion A., Nasirzadeh Y., Lingegowda H., Miller J.E., Khalaj K., Ahn S., Monsanto S.P., Bidarimath M., Sisnett D.J., Craig A.W., Young S.L., Lessey B.A., Koti M., Tayade C. Estrogen mediates inflammatory role of mast cells in endometriosis pathophysiology. Front. Immunol., 2022, Vol. 13, 961599. doi: 10.3389/fimmu.2022.961599.
- Na Z., Guo W., Song J., Feng D., Fang Y., Li D. Identification of novel candidate biomarkers and immune infiltration in polycystic ovary syndrome. J. Ovarian Res., 2022, Vol. 15, no. 1, 80. doi: 10.1186/s13048-022-01013-0.
- Nottola S.A., Makabe S., Stallone T., Macchiarelli G., Correr S., Motta P.M. Ultrastructure and distribution of interstitial glandular cells and associated elements in human fetal ovaries. Arch. Histol. Cytol., 2000, Vol. 63, no. 4, pp. 345-355.
- Theoharides T.C. Neuroendocrinology of mast cells: Challenges and controversies. Exp. Dermatol., 2017, Vol. 26, no. 9, pp. 751-759.
- Thibault C., Levasseur M.C. Ovulation. Am. J. Hum Reprod., 1988, Vol. 3, no. 4, pp. 513-523.
- Zatterale F., Longo M., Naderi J., Raciti G.A., Desiderio A., Miele C., Beguinot F. Chronic Adipose Tissue Inflammation Linking Obesity to Insulin Resistance and Type 2 Diabetes. Front. Physiol., 2020, Vol. 10, 1607. doi: 10.3389/fphys.2019.01607.
- Zdor V.V., Geltser B.I., Eliseikina M.G., Markelova E.V., Tikhonov Y.N., Plekhova N.G., Karaulov A.V. Roles of thyroid hormones, mast cells, and inflammatory mediators in the initiation and progression of autoimmune thyroid diseases. Int. Arch. Allergy Immunol., 2020, Vol. 181, no. 9, pp. 715-726.
Supplementary files
