Features of proinflammatory activation of macrophages in patients with rheumatoid arthritis and systemic lupus erythematosus
- Authors: Bogatyreva A.I.1, Kiseleva D.G.1,2, Cherednichenko V.R.1, Markina Y.V.1, Kirichenko T.V.1
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Affiliations:
- Petrovsky National Research Centre of Surgery
- Lomonosov Moscow State University
- Issue: Vol 27, No 4 (2024)
- Pages: 1057-1064
- Section: SHORT COMMUNICATIONS
- URL: https://journals.rcsi.science/1028-7221/article/view/267927
- DOI: https://doi.org/10.46235/1028-7221-16673-FOP
- ID: 267927
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Abstract
Autoimmune rheumatic diseases (ARDs) are chronic pathological conditions that arise from an abnormal immune response and are accompanied by systemic inflammation. The most common ARDs include rheumatoid arthritis (RA), systemic lupus erythematosus (SLE) and systemic sclerosis (SSc). The exact pathogenesis of ARDs remains unclear, but the complex influence of genetic, immunological and external environmental factors leads to the occurrence and further progression of ARDs. It has been shown that the cause of chronic inflammation may be proinflammatory activation of macrophages, in which an increase in the secretion of cytokines is observed. The aim of this study was to evaluate the inflammatory response of macrophages in patients with RA, SLE and SSc. Materials and methods. The study included 143 participants: 47 patients with RA, 45 patients with SLE, 34 patients with SSc, and 17 people without ARDs and other chronic diseases. Isolation of a primary culture of monocytes was carried out by centrifugation in a ficoll gradient using magnetic separation from the whole blood of study participants. Lipopolysaccharide (LPS) was added to stimulate cells along the proinflammatory pathway. Cell cultivation was carried out for 24 hours. Determination of basal and LPS-stimulated secretion of IL-8 by macrophages was carried out in the culture fluid using an enzyme-linked immunosorbent assay (ELISA). Proinflammatory activation of macrophages was calculated as the ratio of LPS-stimulated and basal IL-8 secretion. Research results. Basal secretion of IL-8 by macrophages was statistically significantly higher in the groups of patients with RA and SSc compared with the SLE and control groups. LPS-stimulated secretion of IL-8 by macrophages in the SSc group had statistically higher values compared to the RA and SLE groups. Proinflammatory activation of macrophages was reduced in the group of patients with RA compared to patients with SLE and the control group, and was also statistically significantly lower in patients with SSc compared to the control group.
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##article.viewOnOriginalSite##About the authors
A. I. Bogatyreva
Petrovsky National Research Centre of Surgery
Author for correspondence.
Email: nastya.bogatyreva.96@mail.ru
Research Associate, Laboratory of Cellular and Molecular Pathology of the Cardiovascular System
Russian Federation, 2 Abrikosovsky Lane, Moscow, 119435D. G. Kiseleva
Petrovsky National Research Centre of Surgery; Lomonosov Moscow State University
Email: nastya.bogatyreva.96@mail.ru
Junior Research Associate, Department of Biophysics, Faculty of Biology, Lomonosov Moscow State University; Junior Research Associate, Laboratory of Cellular and Molecular Pathology of the Cardiovascular System, Petrovsky National Research Centre of Surgery
Russian Federation, 2 Abrikosovsky Lane, Moscow, 119435; MoscowV. R. Cherednichenko
Petrovsky National Research Centre of Surgery
Email: nastya.bogatyreva.96@mail.ru
Junior Research Associate, Laboratory of Cellular and Molecular Pathology of the Cardiovascular System
Russian Federation, 2 Abrikosovsky Lane, Moscow, 119435Yu. V. Markina
Petrovsky National Research Centre of Surgery
Email: nastya.bogatyreva.96@mail.ru
PhD (Medicine), Senior Research Associate, Laboratory of Cellular and Molecular Pathology of the Cardiovascular System
Russian Federation, 2 Abrikosovsky Lane, Moscow, 119435T. V. Kirichenko
Petrovsky National Research Centre of Surgery
Email: nastya.bogatyreva.96@mail.ru
PhD (Medicine), Senior Research Associate, Laboratory of Cellular and Molecular Pathology of the Cardiovascular System
Russian Federation, 2 Abrikosovsky Lane, Moscow, 119435References
- Ahamada M.M., Jia Y., Wu X. Macrophage polarization and plasticity in systemic lupus erythematosus. Front. Immunol., 2021, Vol. 12, 734008. doi: 10.3389/fimmu.2021.734008.
- Al-Adwi Y., Westra J., Goor H., Burgess J.K., Denton C.P., Mulder D.J. Macrophages as determinants and regulators of fibrosis in systemic sclerosis. Rheumatology (Oxford), 2023, Vol. 62, no. 2, 535. doi: 10.1093/rheumatology/keac410.
- Alves L.C.V., Carvalho M.G., Nunes F.F.C., Reis E.A., Ferreira G.A., Calderaro D.C., Carvalho J.S., Pádua P.M., Cicarini W.B., Gondim I.M., Ferreira L.F., Guimarães T.M.P.D., Toledo V.P.C.P. Evaluation of potential biomarkers for the diagnosis and monitoring of Systemic Lupus Erythematosus using the Cytometric Beads Array (CBA). Clin. Chim. Acta, 2019, Vol. 499, pp. 16-23.
- Borzęcka K., Płóciennikowska A., Björkelund H., Sobota A., Kwiatkowska K. CD14 Mediates Binding of High Doses of LPS but Is Dispensable for TNF-α Production. Mediators Inflamm., 2013, Vol. 2013, 824919. doi: 10.1155/2013/824919.
- Carvalheiro T., Horta S., Roon J.A.G., Santiago M., Salvador M.J., Trindade H., Radstake T.R.D.J., Silva J.A.P., Paiva A. Increased frequencies of circulating CXCL10-, CXCL8- and CCL4-producing monocytes and Siglec-3-expressing myeloid dendritic cells in systemic sclerosis patients. Inflamm. Res., 2018, Vol. 67, no. 2, pp. 169-177.
- Gremese E., Tolusso B., Bruno D., Perniola S., Ferraccioli G., Alivernini S. The forgotten key players in rheumatoid arthritis: IL-8 and IL-17 – Unmet needs and therapeutic perspectives. Front. Med. (Lausanne), 2023, Vol. 10, 956127. doi: 10.3389/fmed.2023.956127.
- Koper-Lenkiewicz O.M., Sutkowska K., Wawrusiewicz-Kurylonek N., Kowalewska E., Matowicka-Karna J. Proinflammatory Cytokines (IL-1, -6, -8, -15, -17, -18, -23, TNF-α) Single Nucleotide Polymorphisms in Rheumatoid Arthritis-A Literature Review. Int. J. Mol. Sci., 2022, Vol. 23, no. 4. doi: 10.3390/ijms23042106.
- Kuuliala K., Kuuliala A., Hämäläinen M., Koivuniemi R., Kautiainen H., Moilanen E., Repo H., Leirisalo-Repo M. Impaired akt phosphorylation in monocytes of patients with rheumatoid arthritis. Scand. J. Immunol., 2017, Vol. 85, no. 2, pp. 155-161.
- Liu E., Perl A. Pathogenesis and treatment of autoimmune rheumatic diseases. Curr. Opin. Rheumatol., 2019, Vol. 31, no. 3, pp. 307-315.
- Miller F.W. The increasing prevalence of autoimmunity and autoimmune diseases: an urgent call to action for improved understanding, diagnosis, treatment, and prevention. Curr. Opin. Immunol., 2023, Vol. 80, 102266. doi: 10.1016/j.coi.2022.102266.
- Morita T., Shima Y., Fujimoto K., Tsuboi H., Saeki Y., Narazaki M., Ogata A., Kumanogoh A. Anti-receptor activator of nuclear factor κB ligand antibody treatment increases osteoclastogenesis-promoting IL-8 in patients with rheumatoid arthritis. Int. Immunol., 2019, Vol. 31, no. 5, pp. 277-285.
- Scherer H.U., Häupl T., Burmester G.R. The etiology of rheumatoid arthritis. J. Autoimmun., 2020, Vol. 110, 102400. doi: 10.1016/j.jaut.2019.102400.
- Wallace D.J., Gavin I.M., Karpenko O., Barkhordar F., Gillis B.S. Cytokine and chemokine profiles in fibromyalgia, rheumatoid arthritis and systemic lupus erythematosus: a potentially useful tool in differential diagnosis. Rheumatol. Int., 2015, Vol. 35, no. 6, pp. 991-996.
- Xiang M., Wang Y., Gao Z., Wang J., Chen Q., Sun Z., Liang J., Xu J. Exploring causal correlations between inflammatory cytokines and systemic lupus erythematosus: A Mendelian randomization. Front. Immunol., 2023, Vol. 13, 985729. doi: 10.3389/fimmu.2022.985729.
- Yang S., Zhao M., Jia S. Macrophage: Key player in the pathogenesis of autoimmune diseases. Front. Immunol., 2023, Vol. 14, 1080310. doi: 10.3389/fimmu.2023.1080310.
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