Dynamic features of the humoral immunity in patients with acute coronary syndrome depending on the content of B lymphocytes with the CD3-CD19+CD5+ phenotype, who have and have not COVID-19
- Authors: Safronova E.A.1,2
-
Affiliations:
- South Ural State Medical University
- University of Innovation and Continuing Education of the State Research Center – Burnazyan Federal Medical Biophysical Center, Federal Medical Biological Agency
- Issue: Vol 27, No 2 (2024)
- Pages: 267-272
- Section: SHORT COMMUNICATIONS
- URL: https://journals.rcsi.science/1028-7221/article/view/263686
- DOI: https://doi.org/10.46235/1028-7221-16829-DFO
- ID: 263686
Cite item
Full Text
Abstract
The purpose of the work was to study the dynamic features of the humoral immunity in patients with acute coronary syndrome depending on the content of B lymphocytes with the CD3-CD19+CD5+ phenotype, who had and did not have COVID-19. We analyzed data on humoral immunity in men aged 40 to 65 years with acute coronary syndrome, who had and did not have COVID-19, depending on the content of B lymphocytes with the CD3-CD19+CD5+ phenotype. All patients underwent coronary angiography with further stenting of the coronary arteries. The values of lymphocytes with the CD45+CD3-CD19+ phenotype and the presence or absence of COVID-19 previously were taken into account. Using the flow cytometry method, 2 groups were gated using the pan-leukocyte marker CD45+: the subgroup of B lymphocytes CD3-CD19+CD5+ and the subgroup of B lymphocytes CD3-CD19+CD5-, B lymphocytes (CD45+CD3-CD19+). Levels of total IgA, IgG, IgM, C1-inhibitor, C3a and C5a complement components by enzyme immunoassay.
After coronary artery stenting, B lymphocytes (CD3-CD19+CD5+) significantly increased in the group of people with COVID-19, with initially low levels of these cells (p < 0.01) and normal cells (p < 0.05). In individuals who had COVID-19 and did not have this disease, with initially decreased CD3-CD19+CD5+ cells, B lymphocytes (CD45) increased significantly (p < 0.001 and p < 0.05, respectively) over time (p < 0.05+CD3-CD19+), B lymphocytes (CD3-CD19+CD5-). In the group of patients who recovered from COVID-19 with normal CD3-CD19+CD5+ cells, the relative number of B-lymphocytes decreased over time (p < 0.01) (CD45+CD3-CD19+) and CD3-CD19+CD5- cells. In persons who had previously suffered from COVID-19, with increased CD3-CD19+CD5+ cells, the number of B-lymphocytes (CD45+) significantly (p < 0.05) decreased over time CD3-CD19+), in those who did not have COVID-19 with high above-mentioned cells, immunoglobulin A significantly (p < 0.05) increased. The most severe clinically was the group of people with low CD3-CD19+CD5+B cells and COVID-19 in the anamnesis.
Full Text
##article.viewOnOriginalSite##About the authors
E. A. Safronova
South Ural State Medical University; University of Innovation and Continuing Education of the State Research Center – Burnazyan Federal Medical Biophysical Center, Federal Medical Biological Agency
Author for correspondence.
Email: safronovaeleonora68@gmail.com
PhD (Medicine), Associate Professor, Department of Polyclinic Therapy and Clinical Pharmacology; Assistant Professor, Department of Therapy
Russian Federation, Chelyabinsk; MoscowReferences
- Добрынина М.А., Ибрагимов Р.В., Крицкий И.С., Верховская М.Д., Мосунов А.А., Сарапульцев Г.П., Зурочка А.В., Зурочка В.А., Сарапульцев А.П., Комелькова М.В., Рябова Л.В., Праскурничий Е.А. Постковидный синдром иммунопатологии. Характеристика фенотипических изменений иммунной системы у постковидных пациентов // Медицинская иммунология, 2023. Т. 25, № 4. С. 791-796. [Dobrynina M.A., Ibragimov R.V., Kritsky I.S., Verkhovskaya M.D., Mosunov A.A., Sarapultsev G.P., Zurochka A.V., Zurochka V.A., Sarapultsev A.P., Komelkova M.V., Ryabova L.V., Praskurnichiy E.A. Post-COVID immunopatology syndrome: characteristics of phenotypical changes in the immune system in post-COVID patients. Meditsinskaya immunologiya = Medical Immunology (Russia), 2023, Vol. 25, no. 4, pp. 791-796]. doi: 10.15789/1563-0625-PCI-2707.
- Зурочка А.В., Хайдуков С.В., Кудрявцев И.В., Черешнев В.А. Проточная цитометрия в медицине и биологии. 2-е изд., доп. и расш. Екатеринбург: РИО УрО РАН, 2014. 576 с. [Zurochka A.V., Khaidukov S.V., Kudryavtsev I.V., Chereshnev V.A. Flow cytometry in medicine and biology. 2nd ed., supplemented and expanded. Yekaterinburg: RIO Ural Branch of the Russian Academy of Sciences, 2014. 576 p.
- Хайдуков С.В., Байдун Л.А., Зурочка А.В., Тотолян А.А. Стандартизованная технология «Исследование субпопуляционного состава лимфоцитов периферической крови с применением проточных цитофлюориметров-анализаторов» // Российский иммунологический журнал, 2014. Т. 8 (17), № 4. С. 974-992. [Khaidukov S.V., Baidun L.A., Zurochka A.V., Totolyan A.A. Standardized technology “Study of the subpopulation composition of peripheral blood lymphocytes using flow cytofluorimeter analyzers. Rossiyskiy immunologicheskiy zhurnal = Russian Journal of Immunology, 2014, Vol. 8 (17), no. 4, pp. 974-992. (In Russ.)]
- Casarotti A.C.A., Teixeira D., Longo-Maugeri I.M., Ishimura M.E., Coste M.E.R., Bianco H.T., Moreira F.T., Bacchin A.F., Izar M.C., Gonçalves I., Caixeta A., Szarf G., Pinto I.M., Fonseca F.A. Role of B lymphocytes in the infarcted mass in patients with acute myocardial infarction. Biosci. Rep., 2021, Vol. 41, no. 2, BSR20203413. doi: 10.1042/BSR20203413.
- de Luca L., Rosato S., D’Errigo P., Giordani B., Mureddu G.F., Badoni G., Seccareccia F., Baglio G. Covid-19 diagnosis and mortality in patients with non-ST-elevation myocardial infarction admitted in Italy during the national outbreak. Int. J. Cardiol., 2023, Vol. 1, no. 370, pp. 447-453.
- Esposito L., Cancro F.P., Silverio A., di Maio M., Iannece P., Damato A., Alfano C., de Luca G., Vecchione C., Galasso G. COVID-19 and acute coronary syndromes: from pathophysiology to clinical perspectives. Oxid. Med. Cell. Longev., 2021, Vol. 2021, 4936571. doi: 10.1155/2021/4936571.
- Primorac D., Vrdoljak K., Brlek P., Pavelić E., Molnar V., Matišić V., Erceg Ivkošić I., Parčina M. Adaptive immune responses and immunity to SARS-CoV-2. Front. Immunol., 2022, Vol. 13, 848582. doi: 10.3389/fimmu.2022.848582.
- Sutherland N., Dayawansa N.H., Filipopoulos B., Vasanthakumar S., Narayan O., Ponnuthurai F.A., van Gaal W. Acute coronary syndrome in the COVID-19 pandemic: reduced cases and increased ischaemic time. Heart Lung Circ., 2022, Vol. 31, no. 1, pp. 69-76.
