Mucosal humoral immune response of respiratory tract in medical workers during the post-COVID-19 period
- 作者: Kryukova N.1, Khasanova A.2, Baranova I.1, Kostinov M.3,4, Svitich O.1,3, Chuchalin A.1
-
隶属关系:
- N. Pirogov Russian National Research Medical University
- Ulyanovsk State University
- I. Mechnikov Research Institute of Vaccines and Sera
- First Moscow State I. Sechenov Medical University (Sechenov University)
- 期: 卷 26, 编号 4 (2023)
- 页面: 633-640
- 栏目: Forum Sochi 2023
- URL: https://journals.rcsi.science/1028-7221/article/view/253453
- DOI: https://doi.org/10.46235/1028-7221-13921-MHI
- ID: 253453
如何引用文章
全文:
详细
Currently, the role of local respiratory tract immunoglobulins in COVID-19 and rearrangement of mucosal immune response in the post-COVID period have not been sufficiently studied. Our aim was to evaluate long-term effects of novel coronavirus infection on the mucosal immunity in healthcare workers over the post-infection period.
A total of 180 healthcare workers, ranging in age from 18 to 65 years, were enrolled in a one-stage, cross-sectional study. The subjects with a history of COVID-19 were divided into three groups, depending on the severity of their disease. The control group consisted of 44 healthcare workers who had no history of novel coronavirus infection. Secretory immunoglobulin A (sIgA) and total immunoglobulin G (IgG) levels were quantified in saliva samples, induced sputum samples, naso- and oropharyngeal scrapings by ELISA technique. Specific anti-SARS-CoV-2 IgG antibodies were quantified in the serum by chemiluminescence immunoassay.
Numerous shifts in adaptive immune response were detected for different mucosal compartments, i.e., in subjects who suffered from severe or moderate-to-severe COVID-19, salivary sIgA levels were significantly higher than those in the control group (p < 0.05 and p < 0.005, respectively). An inverse correlation was demonstrated between the levels of total sIgA in all mucosal sites, and the number of days from the onset of disease to the start of study (r = 0.278, р < 0.05). When compared to the control subjects, all the patients with prior COVID-19 had significantly higher levels of total IgG in the induced sputum samples. Total IgG in saliva was also higher in the group of patients who had severe infection (p < 0.05). By contrast, IgG levels in nasopharyngeal samples were decreased in severe and moderately severe groups compared to the control group, thus, probably, indicating an immunodeficiency state in these cases. A direct significant correlation was also detected between the levels of total IgG in all studied samples and the levels of specific IgG antibodies against SARS-CoV-2 in the serum.
Long-term changes in the humoral mucosal immune response were most pronounced in the healthcare workers with a history of severe or moderate-to-severe COVID-19.
作者简介
N. Kryukova
N. Pirogov Russian National Research Medical University
Email: kryukovanadia@gmail.com
Postgraduate Student, Assistant Professor, Department of Hospital Therapy, Pediatric Faculty, N. Pirogov Russian National Research Medical University
俄罗斯联邦, MoscowAlbina Khasanova
Ulyanovsk State University
编辑信件的主要联系方式.
Email: albinafeizer@yandex.ru
ORCID iD: 0000-0003-0301-7548
Postgraduate Student, Department of Infectious Diseases, Ulyanovsk State University
俄罗斯联邦, UlyanovskI. Baranova
N. Pirogov Russian National Research Medical University
Email: iribaranova@yandex.ru
PhD, MD (Medicine), Professor, Department of Hospital Therapy, N. Pirogov Russian National Research Medical University
俄罗斯联邦, MoscowM. Kostinov
I. Mechnikov Research Institute of Vaccines and Sera; First Moscow State I. Sechenov Medical University (Sechenov University)
Email: monolit.96@mail.ru
PhD, MD (Medicine), Professor, Corresponding Member, Russian Academy of Sciences, Head, Department of Epidemiology and Modern Technologies of Vaccination, First Moscow State I. Sechenov Medical University (Sechenov University); Head, Laboratory of Vaccine Prophylaxis and Immunotherapy of Allergy, I. Mechnikov Research Institute of Vaccines and Sera
俄罗斯联邦, Moscow; MoscowO. Svitich
N. Pirogov Russian National Research Medical University; I. Mechnikov Research Institute of Vaccines and Sera
Email: svitichoa@yandex.ru
PhD, MD (Medicine), Professor, Corresponding Member, Russian Academy of Sciences, Director, I. Mechnikov Research Institute of Vaccines and Sera; Professor, Department of Immunology, N. Pirogov Russian National Research Medical University
俄罗斯联邦, Moscow; MoscowA. Chuchalin
N. Pirogov Russian National Research Medical University
Email: pulmomoskva@mail.ru
PhD, MD (Medicine), Professor, Full Member, Russian Academy of Sciences, Head, Department of Hospital Therapy, N. Pirogov Russian National Research Medical University
俄罗斯联邦, Moscow参考
- Временное руководство по лабораторной диагностике COVID-19 в условиях пандемии: Методические рекомендации № 89. М.: ГБУ «НИИОЗММ ДЗМ». [Interim guidance on laboratory diagnosis of COVID-19 in a pandemic: Guidelines No. 89] Moscow: GBU "NIIOZMM DZM".
- Камкин Е.Г., Профилактика, диагностика и лечение новой коронавирусной инфекции (COVID-19). Временные методические рекомендации. М.: Министерство Здравоохранения Российской Федерации, 2020. [Kamkin, E. G. Prevention, Diagnosis and Treatment of Novel Coronavirus Infection (COVID-19). Temporary Guidelines. Ministry of Health of the Russian Federation, 2020.
- Респираторная медицина: руководство: в 3 т. / Под ред. А.Г. Чучалина. 2-е изд., перераб. и доп. М.: Литтера, 2017. Т. 1. C. 148. [Respiratory Medicine: A Manual in 3 Volumes,. Ed. A. Chuchalin. 2nd ed., revised. and additional]. Moscow: Littera, 2017, Vol. 1, P. 148.
- Aita A., Basso D., Cattelan A.M., Fioretto P., Navaglia F., Barbaro F., Stoppa A., Coccorullo E., Farella A., Socal A., Vettor R., Plebania M. SARS-CoV-2 identification and IgA antibodies in saliva: One sample two tests approach for diagnosis. Clin. Chim. Acta, 2020, Vol. 510, pp. 717-722.
- Brandtzaeg P. Secretory immunity with special reference to the oral cavity. J. Oral Microbiol., 2013. Vol. 5. doi: 10.3402/jom.v5i0.20401.
- Guiot J., Demarche S., Henket M., Paulus V., Graff S., Schleich F., Corhay J.-L., Louis R., Moermans C. Methodology for sputum induction and laboratory processing. J. Vis. Exp., 2017, Vol. 130, 56612. doi: 10.3791/56612.
- Isho B., Abe K.T., Zuo M., Jamal A.J., Rathod B., Wang J.H., Li Z., Chao G., Rojas O.L., Bang Y.M., Pu A., Christie-Holmes N., Gervais C., Ceccarelli D., Samavarchi-Tehrani P., Guvenc F., Budylowski P., Li A., Paterson A., Yue F.Y., Marin L.M., Caldwell L., Wrana J.L., Colwill K., Sicheri F., Mubareka S., Gray-Owen S.D., Drews S.J., Siqueira W.L., Barrios-Rodiles M., Ostrowski M., Rini J.M., Durocher Y., McGeer A.J., Gommerman J.L., Gingras A. C. Persistence of serum and saliva antibody responses to SARS-CoV-2 spike antigens in COVID-19 patients. Sci. Immunol., 2020, Vol. 5, no. 52, eabe5511. doi: 10.1126/sciimmunol.abe5511.
- Chao Y.X., Rötzschke O., Tan E.K. The role of IgA in COVID-19. Brain Behav. Immun., 2020, Vol. 87, pp. 182-183.
- Cervia C., Nilsson J., Zurbuchen Y., Valaperti A., Schreiner J., Wolfensberger A., Raeber M.E., Adamo S., Weigang S., Emmenegger M., Hasler S., Bosshard P.P., de Cecco E., Bächli E., Rudiger A., Stüssi-Helbling M., Huber L.C., Zinkernagel A.S., Schaer D.J., Aguzzi A., Kochs G., Held U., Probst-Müller E., Rampini S.K., Boyman O. Systemic and mucosal antibody responses specific to SARS-CoV-2 during mild versus severe COVID-19. J. Allergy Clin. Immunol., 2021, Vol. 147, no. 2, pp. 545-557.
- Pearson C.F., Jeffery R. Mucosal immune responses in COVID19 – a living review. Oxf. Open Immunol., 2021. Vol. 2, no. 1, iqab002. doi: 10.1093/oxfimm/iqab002.
- Fröberg J., Diavatopoulos D.A. Mucosal immunity to severe acute respiratory syndrome coronavirus 2 infection. Curr. Opin. Infect. Dis., 2021. Vol. 34, no. 3, pp. 181-186.
- Russell M. Biological activities of mucosal immunoglobulins. In: J. Mestecky, W. Strober, M.W. Russell, B.L. Kelsall, H. Cheroutre, B.N. Lambrecht, editors. Mucosal Immunology, 4. Amsterdam: Academic Press/Elsevier 2015, pp. 429-454.
- Roda A., Cavalera S., di Nardo F., Calabria D., Rosati S., Simoni P., Colitti B., Baggiani C., Roda M., Anfossi L. Dual lateral flow optical/chemiluminescence immunosensors for the rapid detection of salivary and serum IgA in patients with COVID-19 disease. Biosens. Bioelectron., 2021. Vol. 172, 112765. doi: 10.1016/j.bios.2020.112765.
- Pisanic N., Randad P.R., Kruczynski K., Manabe Y.C., Thomas D., Pekosz A., Klein S.L., Betenbaugh M.J., Clarke W.A., Laeyendecker O., Caturegli P.P., Larman H.B., Detrick B., Fairley J.K., Sherman A.C., Rouphael N., Edupuganti S., Granger D.A., Granger S.W., Collins M., Heaney C.D. COVID-19 Serology at Population Scale: SARS-CoV-2-Specific Antibody Responses in Saliva. J. Clin. Microbiol., 2020, Vol. 59, no. 1, e02204-20. doi: 10.1128/JCM.02204-20.
