sIgA-protease activity of microorganisms isolated from the prostate secretion

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Abstract

Our aim was to characterize the ability of bacteria from prostate secretions of different taxonomic affiliations to inactivate secretory IgA (sIgA). Materials and methods: We performed experiments with 122 isolates of eight types of microorganisms isolated from prostate secretions of the patients with chronic bacterial prostatitis (CBP) and healthy males. The bacterial spectrum of microbiota was studied by bacteriological techniques, the species identification of microorganisms was carried out by mass spectrometry. The sIgA-protease activity of microorganisms was determined by the enzyme immunoassay using the “IgA secretory – ELISA-BEST” kit. Results: A wide prevalence of sIgA-protease activity has been detected in the microorganisms of different types isolated from prostate secretions of patients with CBP and healthy males. E. faecalis was the most active producer of sIgA proteases. We revealed an intraspecific and interspecific variability of the levels of sIgA-protease activity among Staphylococci. The microorganisms isolated from CBP were shown to express a significantly more pronounced ability to inactivate sIgA compared to strains isolated from healthy men.

About the authors

Olga A. Pashinina

Institute of Cellular and Intracellular Symbiosis, Orenburg Federal Research Center, Ural Branch, Russian Academy of Sciences

Author for correspondence.
Email: olga25mikro@mail.ru

PhD (Biology), Senior Research Associate, Laboratory of Microbial Persistence and Symbiosis, Institute of Cellular and Intracellular Symbiosis, Orenburg Federal Research Center, Ural Branch, Russian Academy of Sciences

Russian Federation, Orenburg

O. L. Kartashova

Institute of Cellular and Intracellular Symbiosis, Orenburg Federal Research Center, Ural Branch, Russian Academy of Sciences

Email: labpersist@mail.ru

PhD, MD (Biology), Associate Professor, Leading Research Associate, Laboratory of Microbial Persistence and Symbiosis, Institute of Cellular and Intracellular Symbiosis, Orenburg Federal Research Center, Ural Branch, Russian Academy of Sciences

Russian Federation, Orenburg

T. M. Pashkova

Institute of Cellular and Intracellular Symbiosis, Orenburg Federal Research Center, Ural Branch, Russian Academy of Sciences

Email: labpersist@mail.ru

PhD, MD (Biology), Leading Research Associate, Laboratory of Microbial Persistence and Symbiosis, Institute of Cellular and Intracellular Symbiosis, Orenburg Federal Research Center, Ural Branch, Russian Academy of Sciences

Russian Federation, Orenburg

V. A. Gritsenko

Institute of Cellular and Intracellular Symbiosis, Orenburg Federal Research Center, Ural Branch, Russian Academy of Sciences

Email: vag59@mail.ru

PhD, MD (Medicine), Professor, Chief Research Associate, Institute of Cellular and Intracellular Symbiosis, Orenburg Federal Research Center, Ural Branch, Russian Academy of Sciences

Russian Federation, Orenburg

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2. Figure 1. sIgA-protease activity of microorganisms isolated from prostate secretions in CBP and from healthy men

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Copyright (c) 2023 Pashinina O.A., Kartashova O.L., Pashkova T.M., Gritsenko V.A.

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