Content of myeloid-derived suppressor cells in autoimmune diseases in children

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Abstract

Myeloid-derived suppressor cells (MDSCs) play an important role in regulation of immune response. An increase in their number in adult patients with autoimmune diseases has been reported. G-MDSCs, M-MDSCs, and MDSCs(M-G-) at different stages of autoimmune disease may both activate T cell proliferation, leading to disease progression, or inhibit it, thus promoting Treg differentiation. Arginase-1 (Arg- 1) is an enzyme in MDSCs that reduces the concentration of arginine required for T lymphocyte proliferation. Our aim was to evaluate the content of MDSCs populations and functional activity of MDSCs in children with autoimmune diseases. 75 children with inflammatory bowel diseases (IBD), 60 children with multiple sclerosis (MS), 69 children with psoriasis (PS), 62 healthy age-matched children were included into the study. The content of MDSCs ((CD3, CD19, CD56, HLA-DR)-, CD11b+ and CD33+), subpopulations of MDSCs (M-MDSCs, G-MDSCs expressing CD14 and CD15), assessment of Arg-1 activity were performed by flow cytometry techniques. The content of MDSCs in patients with IBD, MS and PS was significantly higher than in the comparison group and depended on the state of exacerbation/remission. In exacerbation and remission of IBD, MS and PS, a significant increase of MDSCs was revealed when compared with healthy children; the highest values were found in children in exacerbation of MS (Me-3.5 (2.5-5.6) % MNC against Me-1.6 (0.9-2.5) % MNC, p < 0.001). In patients with MS, the content of G-MDSC, M-MDSC was significantly higher, and MDSC(M-G-) was lower than in healthy children. An increase in absolute amounts of G-MDSCs was shown in MS exacerbation compared to the disease remission state (p = 0.022). For patients with IBD, a significant increase in percentage of MDSCs and M-MDSCs (p = 0.014 and p = 0.045, respectively) was obtained in exacerbation of the disease relative to remission state. In patients with IBD, MS, and PS, a significant increase in Arg-1 activity in MDSCs was found, with a decreased number of MDSCs in patients in remission compared to exacerbation phase of the disease. In children with autoimmune diseases, an increase in the MDSC populations was found. The activity of arginase-1 in MDSCs is increased in remission, along with a decrease in their numbers.

About the authors

Tatiana V. Radygina

National Medical Research Center for Children’s Health

Author for correspondence.
Email: tvradigina@mail.ru
ORCID iD: 0000-0003-4704-6885

Ph.D. (Medicine), Senior  Research  Associate, Laboratory of Experimental Immunology and Virology

Russian Federation, 2/1, Lomonosovskiy Pr-t, Moscow, 119991

Daria G. Kuptsova

National Medical Research Center for Children’s Health

Email: dg.kuptsova@gmail.com
ORCID iD: 0000-0001-7771-3314

Junior Researcher, Laboratory of Experimental Immunology and Virology

Russian Federation, 2/1, Lomonosovskiy Pr-t, Moscow, 119991

Svetlana V. Petrichuk

National Medical Research Center for Children’s Health

Email: cito@list.ru
ORCID iD: 0000-0003-0896-6996

PhD, MD (Biology), Professor,  Chief Scientist,  Laboratory of  Experimental Immunology and Virology

Russian Federation, 2/1, Lomonosovskiy Pr-t, Moscow, 119991

Alexander S. Potapov

National Medical Research Center for Children’s Health; I. Sechenov First Moscow State Medical University (Sechenov University)

Email: potapov@nczd.ru
ORCID iD: 0000-0003-4905-2373

PhD, MD (Medicine), Professor, Chief Research Associate, Laboratory of Scientific Foundations of Pediatric Gastroenterology and Hepatology, Head of the Center for Inflammatory Bowel Diseases in Children, Head of Gastroenterology Department with Hepatology Group, National Medical Research Center for Children’s Health, Professor, Department of Pediatrics and Pediatric Rheumatology

Russian Federation, 1/2, Lomonosovskiy Pr-t, Moscow, 119991; 8/2, st. Trubetskaya, Moscow, 119991

Nikolay N. Murashkin

National Medical Research Center for Children’s Health; I. Sechenov First Moscow State Medical University (Sechenov University); Central State Medical Academy of Department of Presidential Affairs

Email: m_nn2001@mail.ru
ORCID iD: 0000-0003-2252-8570

PhD, MD (Medicine), Professor, Head, Research Institute of Pediatric Dermatology, Dermatology Department with Laser Surgery Unit and Children’s Skin Pathology Laboratory, Professor, Department of Dermatovenereology and Cosmetology, Professor, Department of Pediatrics and Pediatric Rheumatology

Russian Federation, 1/2, Lomonosovskiy Pr-t, Moscow, 119991; 8/2, st. Trubetskaya, Moscow, 119991; 19/1A, Marshal Tymoshenko st., Moscow, 19991

Luizat М. Abdullaeva

National Medical Research Center for Children’s Health

Email: instorm@inbox.ru
ORCID iD: 0000-0003-1574-2050

Junior Research assistant, Laboratory of rare hereditary diseases in children of the medical genetic center, neurologist of the Department of Psychoneurology and Psychosomatic Pathology

Russian Federation, 2/1, Lomonosovskiy Pr-t, Moscow, 119991

Olga V. Kurbatova

National Medical Research Center for Children’s Health

Email: putintseva@mail.ru
ORCID iD: 0000-0002-9213-5281

PhD (Medicine), Senior Research Associate, Head, Laboratory of Experimental Immunology and Virology

Russian Federation, 2/1, Lomonosovskiy Pr-t, Moscow, 119991

Valeriya S. Tsvetkova

National Medical Research Center for Children’s Health

Email: tsvetkova.valerie@gmail.com
ORCID iD: 0000-0002-8162-2957

PhD (Medicine), Research Associate, Gastroenterologist, Gastroenterology Department with Hepatology Group

Russian Federation, 2/1, Lomonosovskiy Pr-t, Moscow, 119991

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2. Figure 1. Relative content of MDSCs (A) and Arg-1 activity (B) in patients with IBD, MS, PS during exacerbation (1) and remission (2)

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Copyright (c) 2023 Radygina T.V., Kuptsova D.G., Petrichuk S.V., Potapov A.S., Murashkin N.N., Abdullaeva L.М., Kurbatova O.V., Tsvetkova V.S.

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This work is licensed under a Creative Commons Attribution 4.0 International License.

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