Role of minor lymphocyte populations in development of liver fibrosis in children with glycogen storage disease
- Authors: Kurbatova O.V.1, Petrichuk S.V.1, Movsisyan G.B.1, Kuptsova D.G.1, Radygina T.V.1, Anushenko A.O.1, Semikina E.L.1,2, Potapov A.S.1,2
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Affiliations:
- National Medical Research Center for Children’s Health
- I. Sechenov First Moscow State Medical University
- Issue: Vol 26, No 3 (2023)
- Pages: 337-344
- Section: SHORT COMMUNICATIONS
- URL: https://journals.rcsi.science/1028-7221/article/view/253411
- DOI: https://doi.org/10.46235/1028-7221-10013-ROM
- ID: 253411
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Abstract
Glycogen storage disease (GSD) is a rare condition that changes the way the body uses and stores glycogen. Objective: to evaluate the content of small populations of lymphocytes and their ratios in children with hepatic forms of glycogen disease depending on the stage of liver fibrosis. 148 children with GSD at the age of Me=7,7 [3,9;11,8] were examined. The comparison group consisted of 54 healthy children. The stage of liver fibrosis was carried out on the FibroScan F502 device (EchoSence, France). Immunophenotyping of lymphocytes was performed on CYTOMICS FC500 (Beckman Coulter, USA). Indicators of lymphocyte populations were analyzed as a percentage of deviation from the age norm. In children with GSD, an increase in the degree of liver fibrosis was revealed from age (R=0.57). Treg content in children with GSD was at the lower limit of the age norm and did not depend on the stage of liver fibrosis. The content of Th17 and Thact lymphocytes was significantly higher than that of the comparison group at all stages of liver fibrosis, starting from stage F1. With an increase in the stage of liver fibrosis, there was an increase in the proportion of patients with Thact and Th17 content exceeding the upper limit of the normative values (PF0-F4=0.021 and PF0-F4=0.012, respectively). An increase in Th17/Treg and Thact/Treg ratios was revealed in patients with GSD relative to the comparison group in all age groups, the dynamics of Th17/Treg and Thact/Treg ratios was characterized by their increase with age. Analysis of indicators depending on the stage of liver fibrosis in children with GSD revealed a significant increase in the Thact/Treg ratio from stage F0 to stages F1, F2, F3 and F4 (PF0-F4=0.000). The Th17/Treg index increased from stage F0 to stages F1, F2, F3 (PF0-F3=0.000).
An increase in the content of Thact and Th17 lymphocytes, as well as Th17/Treg and Thact/Treg indices with an increase in the stage of liver fibrosis can be used as an additional tool in assessing fibrotic changes in the liver. Immunological indicators objectively reflect the severity of the patient's condition with hepatic forms of GSD.
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##article.viewOnOriginalSite##About the authors
Olga V. Kurbatova
National Medical Research Center for Children’s Health
Author for correspondence.
Email: putintseva@mail.ru
ORCID iD: 0000-0002-9213-5281
Ph.D. (Medicine) Senior Researcher, Laboratory of Experimental Immunology and Virology
Russian Federation, 2/1 Lomonosovsky Ave, Moscow, 119991Svetlana V. Petrichuk
National Medical Research Center for Children’s Health
Email: cito@list.ru
ORCID iD: 0000-0003-0896-6996
Chief Researcher, Laboratory of Experimental Immunology and Virology
Russian Federation, 2/1 Lomonosovsky Ave, Moscow, 119991Goar B. Movsisyan
National Medical Research Center for Children’s Health
Email: movsisyan@nczd.ru
ORCID iD: 0000-0003-2881-4703
Senior Researcher of the Laboratory of Rare Hereditary Diseases, Gastroenterologist of the Gastroenterology Department with the Hepatological Group
Russian Federation, 2/1 Lomonosovsky Ave, Moscow, 119991Daria G. Kuptsova
National Medical Research Center for Children’s Health
Email: dg.kuptsova@gmail.com
ORCID iD: 0000-0001-7771-3314
Junior Researcher, Clinical Laboratory Physician, Laboratory of Experimental Immunology and Virology
Russian Federation, 2/1 Lomonosovsky Ave, Moscow, 119991T. V. Radygina
National Medical Research Center for Children’s Health
Email: tvradigina@mail.ru
ORCID iD: 0000-0003-4704-6885
Ph.D. (Medicine), Senior Researcher, Laboratory of Experimental Immunology and Virology
Russian Federation, 2/1 Lomonosovsky Ave, Moscow, 119991A. O. Anushenko
National Medical Research Center for Children’s Health
Email: anoushenko@gmail.com
ORCID iD: 0000-0002-1549-2728
Gastroenterologist of the Gastroenterology Department with the Hepatological Group
Russian Federation, 2/1 Lomonosovsky Ave, Moscow, 119991Elena L. Semikina
National Medical Research Center for Children’s Health; I. Sechenov First Moscow State Medical University
Email: semikinaelena@yandex.ru
ORCID iD: 0000-0001-8923-4652
PhD, MD (Medicine), Professor, Chief Research Associate, Laboratory of Research in Pediatric Gastroenterology and Hepatology, Head of Gastroenterology Department with Hepatology Group, Professor, Department of Pediatrics and Pediatric Rheumatology
Russian Federation, 2/1 Lomonosovsky Ave, Moscow, 119991; 8/2, st. Trubetskaya, Moscow, 119991A. S. Potapov
National Medical Research Center for Children’s Health; I. Sechenov First Moscow State Medical University
Email: potapov@nczd.ru
ORCID iD: 0000-0003-4905-2373
PhD, MD (Medicine), Professor, Chief Scientist, Laboratory of scientific foundations of pediatric gastroenterology and hepatology, Head of Gastroenterology Department with Hepatology Group, professor of the Department of Pediatrics and Pediatric Rheumatology
Russian Federation, 2/1 Lomonosovsky Ave, Moscow, 119991; 8/2, st. Trubetskaya, Moscow, 119991References
- Абрамян С.В., Андреев С.Н., Аржанцев А.П., Арутюнов А.С., Афанасьев В.В., Грудянов А.И., Гурин А.Н., Дробышев А.Ю., Дурново Е.А., Панин А.М., Рабинович С.А., Семин В.А.; под редакцией академика РАН А.А. Кулакова. Челюстно-лицевая хирургия: национальное руководство. М.: ГЭОТАР-Медиа, 2019. 691 с. [Abramyan S.V., Andreev S.N., Arzhantsev A.P., Arutyunov A.S., Afanasiev V.V., Grudyanov A.I., Gurin A.N., Drobishev A.Yu., Durnovo E.A., Panin A.M., Rabinovich S.A., Semin V.A.; ed. Academician A.A. Kulakov. Oral and Maxillofacial Surgery: National Guide]. Moscow: GEOTAR-Media, 2019. 691 p.
- Долгушин И.И., Андреева Ю.С., Савочкина А.Ю. Нейтрофильные ловушки и методы оценки функционального статуса нейтрофилов. М.: Издательство РАМН, 2009. 208 с. [Dolgushin I.I., Andreeva Yu.S., Savochkina A.Yu. Neutrophil traps and methods for assessing the functional status of neutrophils]. Moscow: Publishing House of the Russian Academy of Medical Sciences, 2009. 208 p.
- Долгушин И.И., Латюшина Л.С. Влияние местного лечения ронколейкином на течение гнойного раневого процесса и функциональную активность раневых фагоцитов у пациентов с одонтогенными флегмонами // Медицинская иммунология, 2009. Т. 11, № 1. С. 95-100. [Dolgushin I.I., Latyushina L.S. Influence of local treatment with roncoleukin on the course of purulent wound process and the functional activity of wound phagocytes in patients with odontogenic phlegmon. Meditsinskaya immunologiya = Medical Immunology (Russia), 2009, Vol. 11, no. 1, pp. 95-100. (In Russ.)] doi: 10.15789/1563-0625-2009-1-95-100.
- Дурново Е.А. Сравнительный анализ функциональной активности нейтрофилов крови и ротовой полости у больных с гнойно воспалительным процессом в полости рта // Стоматология, 2005. № 3. С.29-32. [Durnovo E.A. Comparative analysis of the functional activity of blood and oral cavity neutrophils in patients with suppurative inflammatory process in the oral cavity. Stomatologiya = Stomatology, 2005, no. 3, pp. 29-32. (In Russ.)]
- Козлов И.Г. Микробиота, мукозальный иммунитет и антибиотики: тонкости взаимодействия // Российский медицинский журнал, 2018. Т. 26, № 8-1. С. 19-27. [Kozlov I.G. Microbiota, mucosal immunity and antibiotics: subtleties of interactio. Rossiyskiy meditsinskiy zhurnal = Medical Journal of the, 2018, Vol. 26, no. 8-1, pp. 19-27. (In Russ.)]
- Латюшина Л.С., Бережная Е.С., Долгушин И.И. Влияние иммунотерапии рекомбинантным IL-1 на клинико-иммунологические показатели пациентов с осложненными переломами нижней челюсти // Проблемы стоматологии, 2017. Т. 13, № 2. С. 49-53. [Latyushina L.S., Berezhnaya E.S., Dolgushin I.I. The effect of immunotherapy with recombinant IL-1 on the clinical and immunological parameters of patients with complicated mandibular fractures. Problemy stomatologii = Dentistry Problems, 2017, Vol. 13, no 2, pp. 49-53. (In Russ.)]
- Латюшина Л.С., Долгушин И.И., Лаптева А.В., Колбина Е.В., Финадеев А.П., Павлиенко Ю.В. Особенности эффекторных показателей фагоцитов и микробиологических параметров локального очага периапикального воспаления при различных формах хронического периодонтита // Проблемы стоматологии, 2021. № 17 (2). С. 96-102. [Latyushina L.S., Dolgushin I.I., Lapteva A.V., Kolbina E.V., Finadeev A.P., Pavlienko Yu.V. Features of the effector parameters of phagocytes and microbiological parameters of the local focus of periapical inflammation in various forms of chronic periodontitis. Problemy stomatologii = Problems of Dentistry, 2021, no. 17 (2), pp. 96-102. (In Russ.)]
- Латюшина Л.С., Алабугин А.В., Насонов А.А., Миндели Н.В., Бобрышева А.Д. Анализ структуры хирургических заболеваний ЧЛО в условиях госпитализации детского и взрослого населения Южного Урала // Проблемы стоматологии. – 2021 № 17 (2). – С. 89-95. [Latyushina L.S., Alabugin A.V., Nasonov A.A., Mindeli N.V., Bobrysheva A.D. Analysis of the structure of surgical diseases of the maxillofacial region in the conditions of hospitalization of children and adults in the Southern Urals. Problemy stomatologii = Problems of Dentistry, 2021, no. 17 (2), pp. 89-95. (In Russ.)]
- Маянский, Д.Н. Хроническое воспаление. М.: Медицина, 1991. 272 с. [Mayansky D.N. Chronic inflammation]. Moscow: Meditsina, 1991. 272 p.
- Маянский А.Н., Маянский Н.А., Заславская М.А. Апоптоз нейтрофилов // Иммунология, 1999. Т. 20, № 6. С. 12-19. [Mayansky A.N., Mayansky N.A., Zaslavskaya M.A. Neutrophil apoptosis Immunologiya = Immunologiya, 1999, Vol. 20, no. 6, pp. 12-19. (In Russ.)]
- Митронин А.В., Робустова Т.Г., Максимовский Ю.М., Лебедев К.А., Понякина И.Д. Клинико-иммунологическая характеристика деструктивных форм хронического периодонтита // Российский стоматологический журнал, 2005. № 1. С. 29-34. [Mitronin A.V., Robustova T.G., Maksimovsky Yu.M., Lebedev K.A., Ponyakina I.D. Clinical and immunological characteristics of destructive forms of chronic periodontitis. Rossiyskiy stomatologicheskiy zhurnal = Russian Dental Journal, 2005, no. 1, pp. 29-34. (In Russ.)]
- Раад З. Результаты непосредственной дентальной имплантации после удаления зубов по поводу хронического периодонтита и тяжелой формы генерализованного пародонтита // Естественные и технические науки, 2009. № 2 (40). С. 164-169. [Raad Z. Results of direct dental implantation after tooth extraction for chronic periodontitis and severe generalized periodontitis. Estestvennye i tekhnicheskie nauki = Natural and Technical Sciences, 2009, no. 2 (40), pp. 164-169. (In Russ.)]
- Ярилин А.А. Основы иммунологии. М.: Медицина, 1999. 602 с. [Yarilin A.A. Fundamentals of immunology]. Moscow: Meditsina, 1999. 602 p.
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