Cooperation of idiotypic and anti-idiotypic antibodies at the steroid-depended chemical carcinogenesis

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Abstract

Immunological research of steroid-depended chemical carcinogenesis in humans is based on positive experience in the clinical usage of selective estrogen receptor modulators and experimental design of immunological methods for human protection from environmental carcinogens. Our study aimed for research of idiotypic antibodies against benzo[a]pyrene, estradiol and progesterone (IgA1-Bp, IgA1-E2 and IgA1-Pg), in connection with anti-idiotypic antibodies specific to estradiol and progesterone (IgG2-E2 and IgG2-Pg) in serum samples of postmenopausal healthy women (HW) and ER+/PR+ stage I breast cancer patients (BCP). Idiotypic antibodies were studied in 402 HW and 475 BCP using ELISA technique, with BP, E2 and Pg conjugated with bovine serum albumin as adsorbed antigens. The anti-idiotypic antibodies were studied using ELISA method and monoclonal antibodies against E2 and Pg as adsorbed antigens. High individual ratios of IgA1-Bp/ IgA1-Pg > 1 and IgA1-E2/IgA1-Pg > 1 were revealed in 42.1% and 48.5% HW, and in 71.1% and 78.1% of BCP cases (p < 0.0001, OR = 5.9 and OR = 3.8, respectively). High IgG2-E2 > 4 levels were found in 23.4% HW and in 41.2% of BCP group (p = 0.0001, OR = 2.3). Combination of IgA1-Bp/IgA1-Pg ≤ 1 with IgG2-E2 ≤ 4 and IgG2-Pg > 2 was more common in HW, than in BCP (29.3% vs 5.8%, p < 0.0001, OR = 0.1). Combinations of IgA1-Bp/IgA1-Pg > 1with IgG2-E2 > 4 or with IgG2-Pg > 2 were more frequent in BCP, than in HW (12.0% and 31.8% vs 4.9% and 15.2%, accordingly, p = 0.01, OR = 2.7 and p = 0.001, OR = 2.6), as well as combination of IgA1-Bp/IgA1-Pg > 1 with IgG2-E2 > 4 and IgG2-Pg > 2 (23.4% vs 9.8%, p = 0.0003, OR = 2.8). Similar specific features were found in HW and BCP when studying IgA1-E2/IgA1-Pg ratio with IgG2-E2 and IgG2-Pg. Nevertheless, high IgA1-Bp/IgA1-Pg > 1 or IgA1-E2/IgA1-Pg > 1 combined with low IgG2-E2 ≤ 4 + IgG2-Pg ≤ 2 were revealed in HW (27.7% and 28.8%) more frequently, than in BCP (19.7%, p = 0.06 and 17.9%, p = 0.008). Excess of IgA1-Bp and IgA1-E2 levels over IgA1-Pg in combination with high IgG2-E2 and IgG2-Pg levels in HW is associated with ER+/PR+ BC stage I condition and may serve as an marker for preventive BC therapy by the targeted ER modulators.

About the authors

Elena G. Polenok

Institute of Human Ecology, Federal Research Center of Coal and Coal chemistry

Email: egpolenok@mail.ru
ORCID iD: 0000-0002-9368-2340
SPIN-code: 3925-0185
Scopus Author ID: 6506567994
ResearcherId: Q-5381-2016

PhD (Pharmacy), Leading Research Associate, Laboratory of Immunochemistry, Institute of Human Ecology, Federal Research Center of Coal and Coal Chemistry

Russian Federation, Kemerovo

Lyudmila A. Gordeeva

Institute of Human Ecology, Federal Research Center of Coal and Coal chemistry

Email: gorsib@rambler.ru
ORCID iD: 0000-0001-5870-7584
SPIN-code: 6662-4616
Scopus Author ID: 14052058500
ResearcherId: R-2781-2016

PhD (Biology), Leading Research Associate, Laboratory of Immunogenetics, Institute of Human Ecology, Federal Research Center of Coal and Coal Chemistry

Russian Federation, Kemerovo

Stella M. Mun

Institute of Human Ecology, Federal Research Center of Coal and Coal chemistry

Author for correspondence.
Email: stellamun@yandex.ru
ORCID iD: 0000-0002-5530-3469
SPIN-code: 5579-8939
Scopus Author ID: 7101645456
ResearcherId: J-6484-2018

PhD (Medicine), Senior Research Associate, Laboratory of Immunogenetics, Institute of Human Ecology, Federal Research Center of Coal and Coal Chemistry

Russian Federation, Kemerovo

Mikhail V. Kostyanko

Institute of Fundamental Sciences, Kemerovo State University

Email: kmvksu@mail.ru
ORCID iD: 0000-0003-0053-1752
SPIN-code: 5953-6554
Scopus Author ID: 6507008191

Leading Engineer, Department of Organic Chemistry, Institute of Fundamental Sciences, Kemerovo State University

Russian Federation, Kemerovo

Alexander V. Antonov

Kuzbass M. Rappoport Clinical Oncology Dispensary

Email: al0412@mail.ru
ORCID iD: 0000-0003-0802-9759

Chief, Breast Cancer Department, Kuzbass M. Rappoport Clinical Oncology Dispensary

Russian Federation, Kemerovo

Natalia E. Verzhbitskaja

Kuzbass Clinical Oncology Dispensary

Email: verzhbitskaia@mail.ru
ORCID iD: 0000-0003-3860-825X

PhD (Medicine), Clinical Pathologist, Pathology Department, Kuzbass M. Rappoport Clinical Oncology Dispensary

Russian Federation, Kemerovo

Pavel V. Bairamov

Kuzbass M. Rappoport Clinical Oncology Dispensary

Email: bayramov_pavel82@mail.ru

Head, Pathology Department, Kuzbass M. Rappoport Clinical Oncology Dispensary

Russian Federation, Kemerovo

Gleb I. Kolpinskiy

Kemerovo State Medical University; Kemerovo Clinical Diagnostic Сenter

Email: Glebss@mail.ru
ORCID iD: 0000-0002-5526-2687
SPIN-code: 6894-6419
Scopus Author ID: 56677706300

PhD, MD (Medicine), Professor, Department of Radiology, Radiotherapy and Oncology, Kemerovo State Medical University; Main Physician, Kemerovo Clinical Diagnostic Сenter

Russian Federation, Kemerovo; Kemerovo

Ilgiz A. Vafin

Kuzbass Center of Blood

Email: gkuz.kots@yandex.ru

Main Physician, Kuzbass Center of Blood

Russian Federation, Kemerovo

Andrey N. Glushkov

Institute of Human Ecology, Federal Research Center of Coal and Coal chemistry

Email: glushkovan@ihe.sbras.ru
ORCID iD: 0000-0002-8560-6719
SPIN-code: 9536-8530
Scopus Author ID: 7006323832
ResearcherId: Q-5985-2016

PhD, MD (Medicine), Professor, Chief Research Associate, Laboratory of Immunogenetics, Institute of Human Ecology, Federal Research Center of Coal and Coal Chemistry

Russian Federation, Kemerovo

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Copyright (c) 2022 Polenok E.G., Gordeeva L.A., Mun S.M., Kostyanko M.V., Antonov A.V., Verzhbitskaja N.E., Bairamov P.V., Kolpinskiy G.I., Vafin I.A., Glushkov A.N.

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