Comparative assessment of genetic polymorphism of Toll-like 2 and 6 receptors predisposing for non-specific ulcerative colitis and irritable bowel syndrome in Russians from Chelyabinsk Region
- Authors: Stashkevich D.S.1, Belyaeva S.V.1, Evdokimov A.V.1
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Affiliations:
- Chelyabinsk State University
- Issue: Vol 25, No 3 (2022)
- Pages: 327-332
- Section: SHORT COMMUNICATIONS
- URL: https://journals.rcsi.science/1028-7221/article/view/120214
- DOI: https://doi.org/10.46235/1028-7221-1139-CAO
- ID: 120214
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Abstract
Ulcerative colitis and irritable bowel syndrome are multifactorial disorders with genetic predisposition. Recent studies suggest that the mucosal immune activation, increased intestinal permeability, and altered host-microbiota interactions may modulate innate immune response, thus contributing to immunopathogenesis of these diseases. Toll-like receptors (TLR) are considered to play the main role in genetic susceptibility to the conditions. The mechanisms for regulating activity of Toll-like receptors are represented by single-nucleotide gene polymorphisms (SNPs), thus producing allelotypes with different biological effects. Among all known TLRs, TLR2 is the most actively studied. The TLR2 gene is located on the long arm of the chromosome 4 and contains the genetic variant leading to the substitution of arginine for glutamine (Arg753Gln) in TLR2 protein. Meanwhile, the most studied SNP of TLR6 is located at the C745T position causing Pro249Ser amino acid substitution in the protein. The present work aimed for analysis of distribution of alleles, genotypes and haplotype combinations of the TLR2 and TLR6 SNPs, and their associations with predisposal for ulcerative colitis and irritable bowel syndrome in Russians from Chelyabinsk Region. The following methods were used in the study: isolation of DNA samples from whole blood, genotyping of the studied gene polymorphisms using PCR with electrophoretic detection. The frequencies of two-locus haplotypes formed by SNPs TLR2 – TLR6 were calculated with Arlequin ver 3.5 software. Comparison of two populational samples for predisposition to UC and IBS was carried out using standard immunogenetic criteria. Significance of differences was set at p ≤ 0.05. Results: Analysis of the data showed the association of specific alleles and genotypes, but not TLR2 – TLR6 haplotypes, with predisposition to the studied diseases. The Arg753Gln gene polymorphism of TLR2 was shown to be significant for a predisposition to ulcerative colitis, and SNP Pro249Ser TLR6 is associated with susceptibility to irritable bowel syndrome in Russians from the Chelyabinsk Region.
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##article.viewOnOriginalSite##About the authors
D. S. Stashkevich
Chelyabinsk State University
Author for correspondence.
Email: stashkevich_dary@mail.ru
PhD (Biology), Associate Professor, Dean, Biological faculty
Russian Federation, ChelyabinskS. V. Belyaeva
Chelyabinsk State University
Email: stashkevich_dary@mail.ru
PhD (Biology), Associate Professor, Department of Microbiology, Immunology and General Biology, Biological faculty
Russian Federation, ChelyabinskA. V. Evdokimov
Chelyabinsk State University
Email: stashkevich_dary@mail.ru
PhD (Biology), Associate Professor, Department of Microbiology, Immunology and General Biology, Biological faculty
Russian Federation, ChelyabinskReferences
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