Study of relationships between maternal HLA-G gene polymorphism and intrauterine infection with risk of congenital malformations
- Authors: Gordeeva L.A.1, Voronina E.N.2, Gareeva Y.V.3, Polenok E.G.1, Mun S.A.1, Glushkov A.N.1
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Affiliations:
- Federal Research Center of Coal and Coal Chemistry, Siberian Branch, Russian Academy of Sciences
- Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences
- LLC "Medical Practice"
- Issue: Vol 24, No 3 (2021)
- Pages: 381-386
- Section: SHORT COMMUNICATIONS
- URL: https://journals.rcsi.science/1028-7221/article/view/120087
- DOI: https://doi.org/10.46235/1028-7221-1041-SOR
- ID: 120087
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Abstract
This study aims for assessing relationships between maternal HLA-G gene polymorphisms (rs41551813, rs12722477, rs41557518) and intrauterine infection with the risk of congenital malformations (CM) in infants. We studied 331 women who had offspring with CMs, and 408 women with one or more healthy children. Influence of the intrauterine infection was analyzed on the basis of laboratory tests. Diagnostics of bacterial vaginosis and vulvovaginal candidiasis by microscopic examination were conducted. Viral infections (herpes simplex virus type 2, cytomegalovirus, human papilloma virus type 16/18) as well as Neisseria gonorrhoeae, Chlamydia trachomatis, Mycoplasma hominis, Mycoplasma genitalium, Ureaplasma urealyticum, Gardnerella vaginalis; Trichomonas vaginalis and Toxoplasma gondii were detected by enzyme-linked immunoassay or polymerase chain reaction (PCR) techniques. The data were obtained from the medical cards of the surveyed women. The gene polymorphisms were typed for Thr31Ser (rs41551813, HLA-G*01:03) in exon 2, Leu110Ile (rs12722477, HLA-G*01:04) and 1597 delС (rs41557518, HLA-G*01:05N) in exon 3 HLA-G using real-time PCR followed by melting analysis. The study showed that maternal age was not a significant risk factor for CMs in the fetus/newborns. Meanwhile, the maternal intrauterine infections were shown to be a significant risk factor for CMs in their infants (OR = 1.57 (1.08-2.29); p = 0.002). It was found that the 110 Ile allele (HLA-G *01:04) was a risk factor for CMs incidence in the fetus/newborns (OR = 1.57 (1.08-2.29), p = 0.01). No association was found between the maternal rs41551813 and rs41557518 HLA-G genetic polymorphisms and CMs in the infants. Hence, intrauterine infections and maternal 110 Ile allele (HLA-G *01:04) may be suggested as risk factors for birth defects in the children. Our results will be useful in understanding the molecular mechanisms of immune disorders in feto-maternal interface.
About the authors
L. A. Gordeeva
Federal Research Center of Coal and Coal Chemistry, Siberian Branch, Russian Academy of Sciences
Author for correspondence.
Email: gorsib@rambler.ru
ORCID iD: 0000-0001-5870-7584
PhD (Biology), Leading Research Associate, Laboratory of Immunogenetics
650065, Kemerovo, Leningradsky ave., 10
Russian FederationE. N. Voronina
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences
Email: voronina_l@mail.ru
PhD (Biology), Research Associate, Molecular Genetics Group
Novosibirsk
Russian FederationYu. V. Gareeva
LLC "Medical Practice"
Email: gareeva.j@bk.ru
Clinical Neonatologist
Kemerovo
Russian FederationE. G. Polenok
Federal Research Center of Coal and Coal Chemistry, Siberian Branch, Russian Academy of Sciences
Email: egpolenok@mail.ru
PhD (Pharmacy), Leading Research Associate, Laboratory of Immunochemistry
650065, Kemerovo, Leningradsky ave., 10
Russian FederationS. A. Mun
Federal Research Center of Coal and Coal Chemistry, Siberian Branch, Russian Academy of Sciences
Email: stellamun@yandex.ru
PhD (Medicine), Senior Research Associate, Laboratory of Immunogenetics
650065, Kemerovo, Leningradsky ave., 10
Russian FederationA. N. Glushkov
Federal Research Center of Coal and Coal Chemistry, Siberian Branch, Russian Academy of Sciences
Email: ihe@kemtel.ru
PhD, MD (Medicine), Deputy Director
650065, Kemerovo, Leningradsky ave., 10
Russian FederationReferences
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