Morphogenetic activity of human brain neuroepithelium during early neurulation
- Authors: Gulimova V.I.1, Saveliev S.V.1, Proshchina A.E.1, Otlyga D.A.1, Sonin G.A.1
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Affiliations:
- Petrovsky National Research Centre of Surgery
- Issue: Vol 164, No 1 (2026)
- Pages: 66-74
- Section: Original Study Articles
- URL: https://journals.rcsi.science/1026-3543/article/view/373767
- DOI: https://doi.org/10.17816/morph.684876
- EDN: https://elibrary.ru/CXUPFN
- ID: 373767
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Abstract
BACKGROUND: During the embryonic period, from the development of the first neural folds to the formation of the primary closure zone of their edges, the neural plate undergoes active transformations. According to general concepts, the first structural signs of nervous system formation appear during neurulation at Carnegie stage 8. At stage 10, the neural plate begins to transform into a tube. During neurulation, the developing elements of the nervous system are most sensitive to damaging influences; however, this period of embryonic neurogenesis remains the least studied.
AIM: The work aimed to clarify the morphogenetic processes during the early stages of neurulation and to compare the sequence of provisional morphogenetic processes of the neuroepithelium in the region of the human brain.
METHODS: Eighteen human embryos obtained during autopsies of women who had died as a result of accidents were examined. After extraction of cytotrophoblasts from the uterine wall, macroscopic analysis, microdissection, and histological examination of the embryos were performed on serial sections.
RESULTS: The study of neurulation in the cranial region of human embryos revealed previously undescribed morphogenetic transformations of the neuroepithelium, including duplication of neural plate folds, formation of temporary neuroectodermal ridges, and other transient embryonic structures. These human-specific provisional morphogenetic events of the developing brain had remained unidentified, and their nature had not been explored.
CONCLUSION: Early embryonic morphogenesis of the human brain is a complex sequence of characteristic cenogenetic movements of the neuroepithelium. In the cranial portion of the neural plate, temporary (provisional) structures arise, sequentially altering the shape of the brain primordium. The emergence and disappearance of these structures likely reflect hidden mechanisms of positional information encoding that determine the subsequent differentiation of the main divisions of the human brain.
Keywords
About the authors
Victoria I. Gulimova
Petrovsky National Research Centre of Surgery
Author for correspondence.
Email: gulimova@yandex.ru
ORCID iD: 0000-0002-7997-8161
SPIN-code: 3755-9666
Cand. Sci. (Biology)
Russian Federation, MoscowSergey V. Saveliev
Petrovsky National Research Centre of Surgery
Email: embrains@hotmail.com
ORCID iD: 0000-0002-1447-7198
SPIN-code: 2079-6351
Dr. Sci. (Biology), professor
Russian Federation, MoscowAlexandra E. Proshchina
Petrovsky National Research Centre of Surgery
Email: proshchina@yandex.ru
ORCID iD: 0000-0002-0515-8275
SPIN-code: 8899-5104
Dr. Sci. (Biology), assistant professor
Russian Federation, MoscowDmitry A. Otlyga
Petrovsky National Research Centre of Surgery
Email: otlyga@bk.ru
ORCID iD: 0000-0002-6719-3383
SPIN-code: 7593-4951
MD, Cand. Sci. (Medicine)
Russian Federation, MoscowGleb A. Sonin
Petrovsky National Research Centre of Surgery
Email: glebs0nin@yandex.ru
ORCID iD: 0000-0001-6844-700X
SPIN-code: 5722-7347
Russian Federation, Moscow
References
- Müller F, O’Rahilly R. The first appearance of the major divisions of the human brain at stage 9. Anat Embryol (Berl). 1983;168(3):419–432. doi: 10.1007/bf00304278
- Müller F, O’Rahilly R. Somitic-vertebral correlation and vertebral levels in the human embryo. Am J Anat. 1986;177(1):3–19. doi: 10.1002/aja.1001770103
- Müller F, O’Rahilly R. The development of the human brain and the closure of the rostral neuropore at stage 11. Anat Embryol (Berl). 1986;175(2):205–222. doi: 10.1007/bf00389597
- Müller F, O’Rahilly R. The development of the human brain, the closure of the caudal neuropore, and the beginning of secondary neurulation at stage 12. Anat Embryol (Berl). 1987;176(4):413–430. doi: 10.1007/BF00310083
- O’Rahilly R, Müller F. The first appearance of the human nervous system at stage 8. Anat Embryol (Berl). 1981;163(1):1–13. doi: 10.1007/bf00315766
- Müller F, O’Rahilly R. The development of the human brain from a closed neural tube at stage 13. Anat Embryol (Berl). 1988;177(3):203–224. doi: 10.1007/bf00321132
- O’Rahilly R, Müller F. The embryonic human brain: An atlas of developmental stages. 3rd ed. Hoboken: Wiley-Liss; 2006. doi: 10.1002/0471973084 ISBN: 9780471973089 EDN: SQSMHD
- Hill MA. Early human development. Clin Obstet Gynecol. 2007;50(1):2–9. doi: 10.1097/GRF.0b013e31802f119d
- O’Rahilly R, Müller F. Developmental stages in human embryos: revised and new measurements. Cells Tissues Organs. 2010;192(2):73–84. doi: 10.1159/000289817
- Copp AJ. Neurulation in the cranial region--normal and abnormal. J Anat. 2005;207(5):623–635. doi: 10.1111/j.1469-7580.2005.00476.x
- Darnell D, Gilbert SF. Neuroembryology. Wiley Interdiscip Rev Dev Biol. 2017;6(1):10.1002/wdev.215. doi: 10.1002/wdev.215
- Nikolopoulou E, Galea GL, Rolo A, et al. Neural tube closure: cellular, molecular and biomechanical mechanisms. Development. 2017;144(4):552–566. doi: 10.1242/dev.145904 EDN: YZZDHZ
- Karzbrun E, Khankhel AH, Megale HC, et al. Human neural tube morphogenesis in vitro by geometric constraints. Nature. 2021;599(7884):268–272. doi: 10.1038/s41586-021-04026-9 EDN: GLVBOM
- Saveliev SV. Stages of embryonic development of the human brain. Moscow: VEDI; 2002. (In Russ.) ISBN: 5-94624-007-2 EDN: YTFZID
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