The Intra-Annual Variability of Immune System Organs and Lysozyme Content in Male Pike Esox lucius L.

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The effect of the physiological state on the lysozyme concentration in the liver, kidney, spleen and serum, as well as the total amount of the enzyme in the organs, was studied in male pike Esox lucius L., 1758 under different conditions of the annual cycle. Multidirectional intra-annual dynamics of organ somatic indices and quantitative parameters of lysozyme in fish tissues and organs, depending on the temperature regime of the water body, was found. Despite significant changes in liver size and lysozyme concentration, the total amount of the enzyme in the organ, the lysozymic index, remained constant. A similar result was found in the spleen. When the influence of the temperature factor at low temperatures was minimized, the liver parameters changed to the opposite ones, while those in the kidney remained unchanged. Such a temperature regime did not affect the spleen parameters and the concentration of the enzyme in the serum. With significant differences between the liver and spleen in the enzyme concentration and size, their lysozymic indices were indicators of the same level. Stages of gonad maturity affected the liver index and the amount of lysozyme in the kidney, but the correlation of the kidney enzyme with maturity was not significant. The kidney lysozyme and the spleen parameters correlated with body mass without organs, although fish weight and length were not influencing factors. The size of the kidney, unlike the spleen, did not affect the enzyme in fish tissues. The liver and kidney enzyme concentrations and kidney lysozymic index were inversely correlated with liver size. A complex nature of the enzyme relationships in organs was revealed, at which the amount of lysozyme in one organ could affect the amount of the enzyme in the other. The relationship between the concentration of serum lysozyme and the morphophysiological parameters of fish under stable low temperature conditions in the water body was not found.

作者简介

M. Subbotkin

Papanin Institute of Inland Water Biology, Russian Academy of Sciences

编辑信件的主要联系方式.
Email: smif@ibiw.ru
Russia, 152742, Nekouzskii district, Yaroslavl oblast, Borok

T. Subbotkina

Papanin Institute of Inland Water Biology, Russian Academy of Sciences

Email: smif@ibiw.ru
Russia, 152742, Nekouzskii district, Yaroslavl oblast, Borok

参考

  1. Буторин Н.В., Курдина Т.Н., Бакастов С.С. Температура воды и грунтов Рыбинского водохранилища. Л.: Наука, 1982. 224 с.
  2. Куровская Л.Я., Стрилько Г.А. Влияние рН водной среды на уровень заражения эктопаразитами, содержание белка и лизоцима у некоторых видов карповых рыб (Cyprinidae) // Рибогосподарська наука Украiни. 2016. Т. 35. № 1. С. 88–101.
  3. Сакун О.Ф., Буцкая Н.А. Определение стадий зрелости и изучение половых циклов рыб // Рыбное хоз-во. 1963. 36 с.
  4. Субботкин М.Ф., Субботкина Т.А. Влияние заражения и инъекций субстанций различной природы на лизоцим карповых рыб (Cyprinidae) (Обзор) // Биол. внутр. вод. 2020. № 2. С. 180–191.
  5. Субботкина Т.А., Субботкин М.Ф. Содержание лизоцима в органах и сыворотке крови у различных видов рыб р. Волги // Журн. эволюционной биохимии и физиол. 2003. Т. 39. № 5. С. 430–437.
  6. Bennett P.M., Janz D.M. Seasonal changes in morphometric and biochemical endpoints in northern pike (Esox lucius), burbot (Lota lota) and slimy sculpin (Cottus cognatus) // Freshwat. Biol. 2007. V. 52. P. 2056–2072.
  7. Cornet V., Khuyen T.D., Mandiki S.N.M., Betoulle S., Bossier P., Reyes–Lopez F.E., Tort L., Kestemont P. GAS1: A new β‑glucan immunostimulant candidate to increase rainbow trout (Oncorhynchus mykiss) resistance to bacterial infections with Aeromonas salmonicida achromogenes // Front. Immunol. 2021. V. 12. 693613.
  8. Crowe B.H., Harris J.O., Bansemer M.S., Stone D.A.J. Restricted feeding and dietary energy levels affect liver structure in cultured Yellowtail Kingfish (Seriola lalandi, Valenciennes) at summer water temperatures // Aquacult. Res. 2021. V. 52. P. 6074–6086.
  9. Feng L., Huang H.-H., Liu Y., Jiang J., Jiang W.-D., Hu K., Li S.-H., Zhou X.-Q. Effect of dietary thiamin supplement on immune responses and intestinal microflora in juvenile Jian carp (Cyprinus carpio var. Jian) // Aquac. Nutr. 2011. V. 17. P. 557–569.
  10. Hansson T., Lindesjoo E., Forlin L., Balk L., Bignert A., Larsson A. Long-term monitoring of the health status of female perch (Perca fluviatilis) in the Baltic Sea shows decreased gonad weight and increased hepatic EROD activity // Aquat. Toxicol. 2006. V. 79. P. 341–355.
  11. Huang J.F., Xu Q.Y., Chang Y.M. Effects of temperature and dietary protein on the growth performance and IGF-I mRNA expression of juvenile mirror carp (Cyprinus carpio) // Aquac. Nutr. 2016. V. 22. № 2. P. 283–292.
  12. Kortet T.R., Taskinen J., Sinisalo T., Jkinen I. Breeding-related seasonal changes in immunocompetence, health state and condition of the cyprinid fish, Rutilus rutilus L. // Biol. J. Linn. Soc. 2003. V. 78. P. 117–127.
  13. Li X., Liu L., Zhang Y., Fang Q., Li Y., Li Y. Toxic effects of chlorpyrifos on lysozyme activities, the contents of complement C3 and IgM, and IgM and complement C3 expressions in common carp (Cyprinus carpio L.) // Chemosphere. 2013. V. 93. P. 428–433.
  14. Medford B.A., Mackay W.C. Protein and lipid content of gonads, liver, and muscle of northern pike (Esox lucius) in relation to gonad growth // J. Fish. Fes. Board Can. 1978. V. 35. P. 213–219.
  15. Miller G.M., Kroon F.J., Metcalfe S., Munday P.L. Temperature is the evil twin: effects of increased temperature and ocean acidification on reproduction in a reef fish // Ecol. Appl. 2015. V. 25. № 3. P. 603–620.
  16. Nguyen M.V., Espe M., Conceição L.E.C., H.M., Yúfera M., Engrola S.A.D., Jordal A.O., Rønnestad I. The role of dietary methionine concentrations on growth, metabolism and N-retention in cobia (Rachycentron canadum) at elevated water temperatures // Aquac. Nutr. 2019. V. 25. № 2. P. 495–507.
  17. Osserman E.F., Lawlor D.P. Serum and urinary lysozyme (Muramidase) in monocytic and monomyelocytic leukemia // J. Exp. Med. 1966. V. 124. P. 921–952.
  18. Qiao Q., Liang H., Zhang X. Effect of cyanobacteria on immune function of crucian carp (Carassius auratus) via chronic exposure in diet // Chemosphere. 2013. V. 90. № 3. P. 1167–1176.
  19. Ren X., Wu B., Zhao F., Qi L., Qiu X., Li R., Yang S., Liu F., Yi G., Ding X., Xia L., Sun Y. Antagonistic activity and protective effect of a Bacillus subtilis isolate against fish pathogen Edwardsiella piscicida // Fisheries Science. 2019. V. 85. P. 1011–1018.
  20. Rohlenova K., Morand S., Hyršl P., Tolarova S., Flajšhans M., Šimkova A. Are fish immune systems really affected by parasites? An immunoecological study of common carp (Cyprinus carpio) // Parasit. Vectors. 2011 V. 4. P. 120.
  21. Subbotkin M.F., Subbotkina T.A. Effect of environment and physiological state of an organism on the activity and content of lysozyme in the fishes of the family Cyprinidae: A review // Inland Water Biol. 2018. V. 11. № 2. P. 184–194.
  22. Sun J.L., Zhao L.L., Liao L., Tang X.H., Cui C., Liu Q., He K., Ma J.D., Jin L., Yan T., Zhou J., Yang S. Interactive effect of thermal and hypoxia on largemouth bass (Micropterus salmoides) gill and liver: Aggravation of oxidative stress, inhibition of immunity and promotion of cell apoptosis // Fish Shellfish Immunol. 2020. V. 98. P. 923–936.
  23. Tarnawska M., Augustyniak M., Łaszczyca P., Migula P., Irnazarow I., Krzyżowski M., Babczyńska A. Immune response of juvenile common carp (Cyprinus carpio L.) exposed to a mixture of sewage chemicals // Fish Shellfish Immunol. 2019. V.88. P. 17–27.
  24. Wang J., Liang D., Yang Q., Tan B., Dong X., Chi S., Liu H., Zhang S. The effect of partial replacement of fish meal by soy protein concentrate on growth performance, immune responses, gut morphology and intestinal inflammation for juvenile hybrid grouper (Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂) // Fish Shellfish Immunol. 2020. V. 98. P. 619–631.
  25. Xu A., Shang-Guan J., Li Zh., Gao Zh., Huang Y., Chen Q. Effects of dietary asafoetida (Ferula sinkiangensis K. M. Shen) levels on feeding attraction activity, growth performance, healthiness, and digestive enzyme activity in juvenile // Fish Physiol. Biochem. 2020. V. 46. P. 1991–2003.

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