Electrochemical Peculiarities of Mediator-Assisted Bioelectrocatalytic Oxidation of Glucose by a New Type of Bioelectrocatalyst
- 作者: Dmitrieva M.1, Gerasimova E.1, Terent’ev A.1, Dobrovol’skii Y.1, Zolotukhina E.1
-
隶属关系:
- Institute of Problems of Chemical Physics, Russian Academy of Sciences
- 期: 卷 55, 编号 9 (2019)
- 页面: 889-899
- 栏目: Article
- URL: https://journals.rcsi.science/1023-1935/article/view/190946
- DOI: https://doi.org/10.1134/S1023193519090064
- ID: 190946
如何引用文章
详细
A protein extract of microbe cells is studied as a bioelectrocatalyst for glucose oxidation. The microbial protein extract prepared from Escherichia coli BB, which comprises all enzymes of the life cycle of these bacteria, is considered here as a model system. This system demonstrates the mediator mechanism of interaction with an inert glassy-carbon electrode in a buffer containing glucose as the substrate. The efficiency of the bioelectrocatalytic process was shown to depend on the type of mediator system and also on the nature of buffer, its temperature, pH, and ionic strength. The protein extract is shown to contain NAD-dependent Fe-glucosodehydrogenase and demonstrate the current densities in mediator-assisted glucose oxidation well comparable with the known data for pure dehydrogenase enzymes and E. coli microbial systems. The prospects for further studies and practical applications of this new bioelectrocatalyst type are outlined.
作者简介
M. Dmitrieva
Institute of Problems of Chemical Physics, Russian Academy of Sciences
编辑信件的主要联系方式.
Email: angel.maria@mail.ru
俄罗斯联邦, Chernogolovka, Moscow oblast, 142432
E. Gerasimova
Institute of Problems of Chemical Physics, Russian Academy of Sciences
Email: angel.maria@mail.ru
俄罗斯联邦, Chernogolovka, Moscow oblast, 142432
A. Terent’ev
Institute of Problems of Chemical Physics, Russian Academy of Sciences
Email: angel.maria@mail.ru
俄罗斯联邦, Chernogolovka, Moscow oblast, 142432
Yu. Dobrovol’skii
Institute of Problems of Chemical Physics, Russian Academy of Sciences
Email: angel.maria@mail.ru
俄罗斯联邦, Chernogolovka, Moscow oblast, 142432
E. Zolotukhina
Institute of Problems of Chemical Physics, Russian Academy of Sciences
Email: angel.maria@mail.ru
俄罗斯联邦, Chernogolovka, Moscow oblast, 142432