Protein-Based Nanobiosensor for Electrochemical Determination of Hydrogen Peroxide


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We designed a hydrogen peroxide biosensor. The electrochemical detection of H2O2 was performed based on immobilization of cobalt nanoparticles–ferritin (CoNPs–Fer) onto multiwalled carbon nanotubes (MWCNTS) ensnared into chitosan (CS) matrices. Electrochemical techniques such as differential pulse voltammetry (DPV) and cyclic voltammetry (CV) was used to check the property of biosensor. Energy-dispersive X-ray spectroscopy (EDXS) and field emission scanning electron microscopy (FESEM) techniques showed the prosperous immobilization of CoNPs–Fer on the modified GC electrode surface. The hydrogen peroxide-designed biosensor displayed a linear range from 0.2 to 14 nM (R2 = 0.99), a detection limit of 1.29 nM (S/N = 3) and sensitivity of –0.1105 μA/nM.The apparent heterogeneous electron transfer rate constant (Ks) and the charge transfer coefficient (α) were gained 4.19 s–1 and 0.49, respectively. This biosensor can detect hydrogen peroxide with high sensitivity, selectivity, and low detection limit.

作者简介

Z. Tamleh

Department of Chemistry, Kermanshah Branch, Islamic Azad University

Email: r.rafipour@iauksh.ac.ir
伊朗伊斯兰共和国, Kermanshah

R. Rafipour

Department of Chemistry, Kermanshah Branch, Islamic Azad University

编辑信件的主要联系方式.
Email: r.rafipour@iauksh.ac.ir
伊朗伊斯兰共和国, Kermanshah

S. Kashanian

Faculty of Chemistry, Sensor and Biosensor Research Center (SBRC) and Nanoscience
and Nanotechnology Research Center (NNRC), Razi University; Nano Drug Delivery Research Center, Kermanshah University of Medical Sciences

Email: r.rafipour@iauksh.ac.ir
伊朗伊斯兰共和国, Kermanshah; Kermanshah

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