Protein-Based Nanobiosensor for Electrochemical Determination of Hydrogen Peroxide
- Авторлар: Tamleh Z.1, Rafipour R.1, Kashanian S.2,3
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Мекемелер:
- Department of Chemistry, Kermanshah Branch, Islamic Azad University
- Faculty of Chemistry, Sensor and Biosensor Research Center (SBRC) and Nanoscience and Nanotechnology Research Center (NNRC), Razi University
- Nano Drug Delivery Research Center, Kermanshah University of Medical Sciences
- Шығарылым: Том 55, № 10 (2019)
- Беттер: 962-969
- Бөлім: Article
- URL: https://journals.rcsi.science/1023-1935/article/view/191031
- DOI: https://doi.org/10.1134/S1023193519100094
- ID: 191031
Дәйексөз келтіру
Аннотация
We designed a hydrogen peroxide biosensor. The electrochemical detection of H2O2 was performed based on immobilization of cobalt nanoparticles–ferritin (CoNPs–Fer) onto multiwalled carbon nanotubes (MWCNTS) ensnared into chitosan (CS) matrices. Electrochemical techniques such as differential pulse voltammetry (DPV) and cyclic voltammetry (CV) was used to check the property of biosensor. Energy-dispersive X-ray spectroscopy (EDXS) and field emission scanning electron microscopy (FESEM) techniques showed the prosperous immobilization of CoNPs–Fer on the modified GC electrode surface. The hydrogen peroxide-designed biosensor displayed a linear range from 0.2 to 14 nM (R2 = 0.99), a detection limit of 1.29 nM (S/N = 3) and sensitivity of –0.1105 μA/nM.The apparent heterogeneous electron transfer rate constant (Ks) and the charge transfer coefficient (α) were gained 4.19 s–1 and 0.49, respectively. This biosensor can detect hydrogen peroxide with high sensitivity, selectivity, and low detection limit.
Негізгі сөздер
Авторлар туралы
Z. Tamleh
Department of Chemistry, Kermanshah Branch, Islamic Azad University
Email: r.rafipour@iauksh.ac.ir
Иран, Kermanshah
R. Rafipour
Department of Chemistry, Kermanshah Branch, Islamic Azad University
Хат алмасуға жауапты Автор.
Email: r.rafipour@iauksh.ac.ir
Иран, Kermanshah
S. Kashanian
Faculty of Chemistry, Sensor and Biosensor Research Center (SBRC) and Nanoscienceand Nanotechnology Research Center (NNRC), Razi University; Nano Drug Delivery Research Center, Kermanshah University of Medical Sciences
Email: r.rafipour@iauksh.ac.ir
Иран, Kermanshah; Kermanshah
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