A hydrogen peroxide biosensor based on horseradish peroxidase/poly(L-leucine)/polydopamine modified glassy carbon electrode
- Authors: Zheng X.1, Guo Y.1, Zheng J.1, Ma C.1, Zhou X.1, Lin J.1, Lin R.1
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Affiliations:
- School of Life Sciences
- Issue: Vol 53, No 5 (2017)
- Pages: 443-451
- Section: Article
- URL: https://journals.rcsi.science/1023-1935/article/view/188671
- DOI: https://doi.org/10.1134/S1023193517050172
- ID: 188671
Cite item
Abstract
A simple and practical sensor of hydrogen peroxide (H2O2) was designed successfully. The mixture of horseradish peroxidase (HRP) and chitosan (Chit) are effectively immobilized on the surface of poly-L-leucine/polydopamine modified glassy carbon electrode (PL-LEU/PDA/GCE). Under the optimum conditions, the biosensor based on HRP exhibits a fast amperometric response (within 3 s) to H2O2. The linear response range of the sensor is 0.5–952.0 μmol L–1, with the detection limit of 0.1 μmol L–1 (S/N = 3) and the sensitivity of 0.23 A L moL–1 cm–2. The apparent Michaelis–Menten constant (kMapp) of the biosensor is evaluated to be 0.12 mmol L–1, which suggests that the HRP-Chit/PL-LEU/PDA/GCE shows a higher affinity for H2O2. The sensor exhibits good sensitivity, selectivity, stability and reproducibility. The proposed method has been successfully applied to the determination of H2O2 in practical samples.
About the authors
Xinyu Zheng
School of Life Sciences
Email: ruiyulin121011@sina.com
China, Fuzhou, 350002
Yuheng Guo
School of Life Sciences
Email: ruiyulin121011@sina.com
China, Fuzhou, 350002
Jinshuai Zheng
School of Life Sciences
Email: ruiyulin121011@sina.com
China, Fuzhou, 350002
Cong Ma
School of Life Sciences
Email: ruiyulin121011@sina.com
China, Fuzhou, 350002
Xuechou Zhou
School of Life Sciences
Email: ruiyulin121011@sina.com
China, Fuzhou, 350002
Jiandi Lin
School of Life Sciences
Email: ruiyulin121011@sina.com
China, Fuzhou, 350002
Ruiyu Lin
School of Life Sciences
Author for correspondence.
Email: ruiyulin121011@sina.com
China, Fuzhou, 350002