Tyrosine sensing on phthalic anhydride functionalized chitosan and carbon nanotube film coated glassy carbon electrode
- Authors: Chekin F.1, Bagheri S.2
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Affiliations:
- Department of Chemistry, Ayatollah Amoli Branch
- Centre for Research in Nanotechnology and Catalysis (NANOCEN), IPS Building
- Issue: Vol 52, No 2 (2016)
- Pages: 174-180
- Section: Article
- URL: https://journals.rcsi.science/1023-1935/article/view/187673
- DOI: https://doi.org/10.1134/S1023193515120034
- ID: 187673
Cite item
Abstract
Phthaloylchitosan (PHCS) has been synthesized by a simple and low-cost method using chitosan and phthalic anhydride as organic precursors by microwave irradiation. Techniques of nuclear magnetic resonance (NMR), FT-IR spectroscopy and transmission electron microscope (TEM) were used to characterize the structure and properties of the Phthaloylchitosan. Moreover, glassy carbon electrode modified with Phthaloylchitosan and carbon nanotube (PHCS–CNT/GCE) was prepared by casting of the PHCS–CNT solution on GCE. The electrochemical behavior of PHCS–CNT/GCE was investigated and compared with the electrochemical behavior of Phthaloylchitosan modified GC (PHCS/GC), carbon nanotube modified GC (CNT/GC) and unmodified GC using cyclic voltammetry (CV). The Phthaloylchitosan film is electrochemically inactive; similar background charging currents are observed at bare GC. Electrochemical parameters, including apparent diffusion coefficient for the Fe(CN)63-/4- redox probe at PHCS–CNT/GCE is comparable to values reported for GCE, CNT/GCE and PHCS/GCE. The PHCS–CNT/GCE sensor responded linearly to tyrosine (Tyr) in the concentration of 1.0 × 10–6 to 8.0 × 10–4 M with detection limit of 3.0 × 10–7 M at 3σ using amperometry. In addition, the PHCS–CNT/GCE displayed good reproducibility, high sensitivity and good selectivity towards the determination of Tyr, making it suitable for the determination of Tyr in clinical and medicine.
About the authors
Fereshteh Chekin
Department of Chemistry, Ayatollah Amoli Branch
Author for correspondence.
Email: fchekin@yahoo.com
Iran, Islamic Republic of, Amol
Samira Bagheri
Centre for Research in Nanotechnology and Catalysis (NANOCEN), IPS Building
Email: fchekin@yahoo.com
Malaysia, Kuala Lumpur, 50603