Interpretation of FISH Results in the Case of Nonuniform Internal Radiation Exposure of Human Body with the Use of Model Approach

详细

The fluorescence in situ hybridization (FISH) technique allows for the estimation of the number of stable chromosomal aberrations (translocations) in human blood T-lymphocytes decades after radiation exposure. Therefore, it is used for retrospective assessment of irradiation doses received by red bone marrow (RBM). Cytogenetic analysis of individuals exposed on the Techa River in the 1950s to ^89,90Sr, locally irradiating RBM, showed that dose assessment based on FISH data led to underestimation of the actual dose estimated from measurements of whole-body ⁹⁰Sr content. The purpose of current study was to evaluate the dose–effect relationship and the frequency of translocations per 1 Gy of RBM irradiation dose and T-lymphocytes dose for donors living in Techa riverside villages (n = 178; translocation yield = 2389; number of genome equivalents GE = 96 446). Irradiation doses were estimated using the latest version of the Techa River dosimetry system and the T-cell irradiation model. Statistical analysis showed that the frequency of translocations per 1000 GE per 1 Gy of RBM dose was 8.0 ± 0.7; this value was statistically significantly lower than the value estimated for Sellafield radiation workers under external exposure (11.6 ± 1.6). At the same time, the use of T-cell and their progenitor doses led to re-evaluation (increase) in the slope of the dose–response curve to 11.8 ± 1.6 per 1 Gy per 1000 GE, which agreed well with the published data. The obtained estimates of the background frequencies of translocations also were close to the published values ​​for unexposed donors. These findings demonstrate the legitimacy of using a model approach for estimation of irradiation doses in T-lymphocytes and their progenitors and the need to take into account complex T-cell dynamics in interpretation of cytogenetic data for the purposes of biodosimetry.