Genome structure and origin of nontoxigenic strains of Vibrio cholerae of El Tor biovar with different epidemiological significance
- 作者: Smirnova N.1, Kul’shan’ T.1, Baranikhina E.1, Krasnov Y.1, Agafonov D.1, Kutyrev V.1
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隶属关系:
- Russian Research Anti-Plague Institute Microbe
- 期: 卷 52, 编号 9 (2016)
- 页面: 914-925
- 栏目: Genetics of Microorganisms
- URL: https://journals.rcsi.science/1022-7954/article/view/187872
- DOI: https://doi.org/10.1134/S1022795416060120
- ID: 187872
如何引用文章
详细
Intraspecies genetic differentiation of nontoxigenic strains of Vibrio cholerae of El Tor biovar containing one of the key pathogenicity genes, tcpA, is studied along with the phylogenetic relationships between these strains and toxigenic isolates. Comparative analysis of the whole genome nucleotide sequences demonstrates for the first time that ctxA–tcpA+ strains vary considerably and can be clustered into two separate groups, the CTXφ–RS1φ+VPI+VSP+/CTXφ–RS1φ–VPI+VSP+ isolates and the CTXφ–RS1φ–VPI+VSP– isolates, differing in their epidemiological significance. In the course of model experiments, it is established that nontoxigenic potentially epidemic CTXφ–RS1φ+VPI+VSP+/CTXφ–RS1φ–VPI+VSP+ isolates are derivatives of toxigenic strains. The results of whole genome SNP analysis of 35 Vibrio cholerae strains confirm these data and indicate genetic remoteness of nontoxigenic CTXφ–RS1φ–VPI+VSP– strains both from the potentially epidemic strains and from the toxigenic isolates. It is found that the genomes of the CTXφ–RS1φ–VPI+VSP– strains contain unique SNPs which are characteristic of them alone. The new data on the structure of the genome of nontoxigenic strains with different epidemiological significance may be further used for their genetic differentiation.
作者简介
N. Smirnova
Russian Research Anti-Plague Institute Microbe
编辑信件的主要联系方式.
Email: rusrapi@microbe.ru
俄罗斯联邦, Saratov, 410005
T. Kul’shan’
Russian Research Anti-Plague Institute Microbe
Email: rusrapi@microbe.ru
俄罗斯联邦, Saratov, 410005
E. Baranikhina
Russian Research Anti-Plague Institute Microbe
Email: rusrapi@microbe.ru
俄罗斯联邦, Saratov, 410005
Ya. Krasnov
Russian Research Anti-Plague Institute Microbe
Email: rusrapi@microbe.ru
俄罗斯联邦, Saratov, 410005
D. Agafonov
Russian Research Anti-Plague Institute Microbe
Email: rusrapi@microbe.ru
俄罗斯联邦, Saratov, 410005
V. Kutyrev
Russian Research Anti-Plague Institute Microbe
Email: rusrapi@microbe.ru
俄罗斯联邦, Saratov, 410005