Genome structure and origin of nontoxigenic strains of Vibrio cholerae of El Tor biovar with different epidemiological significance
- Авторлар: Smirnova N.1, Kul’shan’ T.1, Baranikhina E.1, Krasnov Y.1, Agafonov D.1, Kutyrev V.1
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Мекемелер:
- Russian Research Anti-Plague Institute Microbe
- Шығарылым: Том 52, № 9 (2016)
- Беттер: 914-925
- Бөлім: Genetics of Microorganisms
- URL: https://journals.rcsi.science/1022-7954/article/view/187872
- DOI: https://doi.org/10.1134/S1022795416060120
- ID: 187872
Дәйексөз келтіру
Аннотация
Intraspecies genetic differentiation of nontoxigenic strains of Vibrio cholerae of El Tor biovar containing one of the key pathogenicity genes, tcpA, is studied along with the phylogenetic relationships between these strains and toxigenic isolates. Comparative analysis of the whole genome nucleotide sequences demonstrates for the first time that ctxA–tcpA+ strains vary considerably and can be clustered into two separate groups, the CTXφ–RS1φ+VPI+VSP+/CTXφ–RS1φ–VPI+VSP+ isolates and the CTXφ–RS1φ–VPI+VSP– isolates, differing in their epidemiological significance. In the course of model experiments, it is established that nontoxigenic potentially epidemic CTXφ–RS1φ+VPI+VSP+/CTXφ–RS1φ–VPI+VSP+ isolates are derivatives of toxigenic strains. The results of whole genome SNP analysis of 35 Vibrio cholerae strains confirm these data and indicate genetic remoteness of nontoxigenic CTXφ–RS1φ–VPI+VSP– strains both from the potentially epidemic strains and from the toxigenic isolates. It is found that the genomes of the CTXφ–RS1φ–VPI+VSP– strains contain unique SNPs which are characteristic of them alone. The new data on the structure of the genome of nontoxigenic strains with different epidemiological significance may be further used for their genetic differentiation.
Негізгі сөздер
Авторлар туралы
N. Smirnova
Russian Research Anti-Plague Institute Microbe
Хат алмасуға жауапты Автор.
Email: rusrapi@microbe.ru
Ресей, Saratov, 410005
T. Kul’shan’
Russian Research Anti-Plague Institute Microbe
Email: rusrapi@microbe.ru
Ресей, Saratov, 410005
E. Baranikhina
Russian Research Anti-Plague Institute Microbe
Email: rusrapi@microbe.ru
Ресей, Saratov, 410005
Ya. Krasnov
Russian Research Anti-Plague Institute Microbe
Email: rusrapi@microbe.ru
Ресей, Saratov, 410005
D. Agafonov
Russian Research Anti-Plague Institute Microbe
Email: rusrapi@microbe.ru
Ресей, Saratov, 410005
V. Kutyrev
Russian Research Anti-Plague Institute Microbe
Email: rusrapi@microbe.ru
Ресей, Saratov, 410005