Development of Methods for the Target-Specific Protein Elimination in Plants
- Авторы: Maloshenok L.1, Abdeeva I.1, Panina J.1, Piruzian E.1, Zolotarenko A.1, Bruskin S.1
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Учреждения:
- Vavilov Institute of General Genetics, Russian Academy of Sciences
- Выпуск: Том 54, № 11 (2018)
- Страницы: 1378-1382
- Раздел: Short Communications
- URL: https://journals.rcsi.science/1022-7954/article/view/188691
- DOI: https://doi.org/10.1134/S1022795418110091
- ID: 188691
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Аннотация
Here we present the method for the target-specific elimination of certain intracellular proteins in plants using the ubiquitin-proteasome system. We modified the E3 ubiquitin ligase Chip of A. thaliana to obtain two variants carrying the deletions at the N-terminus and the GFP recognition domain. The interaction of the GFP protein and the chimeric ubiquitin ligase was confirmed via yeast two-hybrid assay. Fluorescence microscopy and fluorimetry showed that, when an infiltration of the gfp-expressing N. benthamiana plants was performed with agrobacteria carrying the hybrid E3 gene of the ubiquitin ligase Chip with the GFP recognition domain, a significant decrease in the fluorescence was observed for both variants: carrying the N-terminal deletion of 100 amino acids or the deletion of 140 amino acids.
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L. Maloshenok
Vavilov Institute of General Genetics, Russian Academy of Sciences
Автор, ответственный за переписку.
Email: maloshenoklg@mail.ru
Россия, Moscow, 119991
I. Abdeeva
Vavilov Institute of General Genetics, Russian Academy of Sciences
Email: maloshenoklg@mail.ru
Россия, Moscow, 119991
J. Panina
Vavilov Institute of General Genetics, Russian Academy of Sciences
Email: maloshenoklg@mail.ru
Россия, Moscow, 119991
E. Piruzian
Vavilov Institute of General Genetics, Russian Academy of Sciences
Email: maloshenoklg@mail.ru
Россия, Moscow, 119991
A. Zolotarenko
Vavilov Institute of General Genetics, Russian Academy of Sciences
Email: maloshenoklg@mail.ru
Россия, Moscow, 119991
S. Bruskin
Vavilov Institute of General Genetics, Russian Academy of Sciences
Email: maloshenoklg@mail.ru
Россия, Moscow, 119991