Development of Methods for the Target-Specific Protein Elimination in Plants
- Авторлар: Maloshenok L.1, Abdeeva I.1, Panina J.1, Piruzian E.1, Zolotarenko A.1, Bruskin S.1
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Мекемелер:
- Vavilov Institute of General Genetics, Russian Academy of Sciences
- Шығарылым: Том 54, № 11 (2018)
- Беттер: 1378-1382
- Бөлім: Short Communications
- URL: https://journals.rcsi.science/1022-7954/article/view/188691
- DOI: https://doi.org/10.1134/S1022795418110091
- ID: 188691
Дәйексөз келтіру
Аннотация
Here we present the method for the target-specific elimination of certain intracellular proteins in plants using the ubiquitin-proteasome system. We modified the E3 ubiquitin ligase Chip of A. thaliana to obtain two variants carrying the deletions at the N-terminus and the GFP recognition domain. The interaction of the GFP protein and the chimeric ubiquitin ligase was confirmed via yeast two-hybrid assay. Fluorescence microscopy and fluorimetry showed that, when an infiltration of the gfp-expressing N. benthamiana plants was performed with agrobacteria carrying the hybrid E3 gene of the ubiquitin ligase Chip with the GFP recognition domain, a significant decrease in the fluorescence was observed for both variants: carrying the N-terminal deletion of 100 amino acids or the deletion of 140 amino acids.
Негізгі сөздер
Авторлар туралы
L. Maloshenok
Vavilov Institute of General Genetics, Russian Academy of Sciences
Хат алмасуға жауапты Автор.
Email: maloshenoklg@mail.ru
Ресей, Moscow, 119991
I. Abdeeva
Vavilov Institute of General Genetics, Russian Academy of Sciences
Email: maloshenoklg@mail.ru
Ресей, Moscow, 119991
J. Panina
Vavilov Institute of General Genetics, Russian Academy of Sciences
Email: maloshenoklg@mail.ru
Ресей, Moscow, 119991
E. Piruzian
Vavilov Institute of General Genetics, Russian Academy of Sciences
Email: maloshenoklg@mail.ru
Ресей, Moscow, 119991
A. Zolotarenko
Vavilov Institute of General Genetics, Russian Academy of Sciences
Email: maloshenoklg@mail.ru
Ресей, Moscow, 119991
S. Bruskin
Vavilov Institute of General Genetics, Russian Academy of Sciences
Email: maloshenoklg@mail.ru
Ресей, Moscow, 119991