Expression of plant antimicrobial peptide pro-SmAMP2 gene increases resistance of transgenic potato plants to Alternaria and Fusarium pathogens
- Авторы: Vetchinkina E.1, Komakhina V.1, Vysotskii D.1, Zaitsev D.2, Smirnov A.2, Babakov A.1, Komakhin R.1
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Учреждения:
- All-Russian Research Institute of Agricultural Biotechnology
- Department of Plant Protection
- Выпуск: Том 52, № 9 (2016)
- Страницы: 939-951
- Раздел: Plant Genetics
- URL: https://journals.rcsi.science/1022-7954/article/view/187877
- DOI: https://doi.org/10.1134/S1022795416080147
- ID: 187877
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Аннотация
The chickweed (Stellaria media L.) pro-SmAMP2 gene encodes the hevein-like peptides that have in vitro antimicrobial activity against certain harmful microorganisms. These peptides play an important role in protecting the chickweed plants from infection, and the pro-SmAMP2 gene was previously used to protect transgenic tobacco and Arabidopsis plants from phytopathogens. In this study, the pro-SmAMP2 gene under control of viral CaMV35S promoter or under control of its own pro-SmAMP2 promoter was transformed into cultivated potato plants of two cultivars, differing in the resistance to Alternaria: Yubiley Zhukova (resistant) and Skoroplodny (susceptible). With the help of quantitative real-time PCR, it was demonstrated that transgenic potato plants expressed the pro-SmAMP2 gene under control of both promoters at the level comparable to or exceeding the level of the potato actin gene. Assessment of the immune status of the transformants demonstrated that expression of antimicrobial peptide pro-SmAMP2 gene was able to increase the resistance to a complex of Alternaria sp. and Fusarium sp. phytopathogens only in potato plants of the Yubiley Zhukova cultivar. The possible role of the pro-SmAMP2 products in protecting potatoes from Alternaria sp. and Fusarium sp. is discussed.
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Об авторах
E. Vetchinkina
All-Russian Research Institute of Agricultural Biotechnology
Автор, ответственный за переписку.
Email: recombination@iab.ac.ru
Россия, Moscow, 127550
V. Komakhina
All-Russian Research Institute of Agricultural Biotechnology
Email: recombination@iab.ac.ru
Россия, Moscow, 127550
D. Vysotskii
All-Russian Research Institute of Agricultural Biotechnology
Email: recombination@iab.ac.ru
Россия, Moscow, 127550
D. Zaitsev
Department of Plant Protection
Email: recombination@iab.ac.ru
Россия, Moscow, 127550
A. Smirnov
Department of Plant Protection
Email: recombination@iab.ac.ru
Россия, Moscow, 127550
A. Babakov
All-Russian Research Institute of Agricultural Biotechnology
Email: recombination@iab.ac.ru
Россия, Moscow, 127550
R. Komakhin
All-Russian Research Institute of Agricultural Biotechnology
Email: recombination@iab.ac.ru
Россия, Moscow, 127550