Expression of plant antimicrobial peptide pro-SmAMP2 gene increases resistance of transgenic potato plants to Alternaria and Fusarium pathogens
- Autores: Vetchinkina E.1, Komakhina V.1, Vysotskii D.1, Zaitsev D.2, Smirnov A.2, Babakov A.1, Komakhin R.1
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Afiliações:
- All-Russian Research Institute of Agricultural Biotechnology
- Department of Plant Protection
- Edição: Volume 52, Nº 9 (2016)
- Páginas: 939-951
- Seção: Plant Genetics
- URL: https://journals.rcsi.science/1022-7954/article/view/187877
- DOI: https://doi.org/10.1134/S1022795416080147
- ID: 187877
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Resumo
The chickweed (Stellaria media L.) pro-SmAMP2 gene encodes the hevein-like peptides that have in vitro antimicrobial activity against certain harmful microorganisms. These peptides play an important role in protecting the chickweed plants from infection, and the pro-SmAMP2 gene was previously used to protect transgenic tobacco and Arabidopsis plants from phytopathogens. In this study, the pro-SmAMP2 gene under control of viral CaMV35S promoter or under control of its own pro-SmAMP2 promoter was transformed into cultivated potato plants of two cultivars, differing in the resistance to Alternaria: Yubiley Zhukova (resistant) and Skoroplodny (susceptible). With the help of quantitative real-time PCR, it was demonstrated that transgenic potato plants expressed the pro-SmAMP2 gene under control of both promoters at the level comparable to or exceeding the level of the potato actin gene. Assessment of the immune status of the transformants demonstrated that expression of antimicrobial peptide pro-SmAMP2 gene was able to increase the resistance to a complex of Alternaria sp. and Fusarium sp. phytopathogens only in potato plants of the Yubiley Zhukova cultivar. The possible role of the pro-SmAMP2 products in protecting potatoes from Alternaria sp. and Fusarium sp. is discussed.
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Sobre autores
E. Vetchinkina
All-Russian Research Institute of Agricultural Biotechnology
Autor responsável pela correspondência
Email: recombination@iab.ac.ru
Rússia, Moscow, 127550
V. Komakhina
All-Russian Research Institute of Agricultural Biotechnology
Email: recombination@iab.ac.ru
Rússia, Moscow, 127550
D. Vysotskii
All-Russian Research Institute of Agricultural Biotechnology
Email: recombination@iab.ac.ru
Rússia, Moscow, 127550
D. Zaitsev
Department of Plant Protection
Email: recombination@iab.ac.ru
Rússia, Moscow, 127550
A. Smirnov
Department of Plant Protection
Email: recombination@iab.ac.ru
Rússia, Moscow, 127550
A. Babakov
All-Russian Research Institute of Agricultural Biotechnology
Email: recombination@iab.ac.ru
Rússia, Moscow, 127550
R. Komakhin
All-Russian Research Institute of Agricultural Biotechnology
Email: recombination@iab.ac.ru
Rússia, Moscow, 127550