Insertional inactivation of virulence operon in population of persistent Bordetella pertussis bacteria


Citar

Texto integral

Acesso aberto Acesso aberto
Acesso é fechado Acesso está concedido
Acesso é fechado Somente assinantes

Resumo

Avirulent B. pertussis bacteria containing IS elements in the bvgAS operon were detected during the study of whooping cough patients and bacilli carriers. The present work is devoted to the study of the accumulation dynamics and the mechanisms of generation of persistent forms of the B. pertussis bacteria in lower monkeys as the most adequate model for extrapolation of the experiment results to humans. By means of the real-time PCR method, it was established that the B. pertussis bacteria lived more than three months in the upper respiratory tract after a single intranasal monkey infection; the period was reduced to 14–28 days during repeated infection. An increase in the portion of B. pertussis Bvg mutants in the population to tens of percent from the total number of registered bacteria was registered. The experimental confirmation of the development and accumulation of avirulent B. pertussis Bvg mutants during the development of the infectious process was obtained. Further study of the composition of the B. pertussis persistent bacteria population at different stages of the disease will make it possible to formulate new approaches to the whooping cough diagnostics and prevention and creation of fundamentally new drugs.

Sobre autores

G. Karataev

Gamaleya Research Center for Epidemiology and Microbiology

Autor responsável pela correspondência
Email: karataevgi@rambler.ru
Rússia, Moscow, 123098

L. Sinyashina

Gamaleya Research Center for Epidemiology and Microbiology

Email: niiepit@rambler.ru
Rússia, Moscow, 123098

A. Medkova

Gamaleya Research Center for Epidemiology and Microbiology

Email: niiepit@rambler.ru
Rússia, Moscow, 123098

E. Semin

Gamaleya Research Center for Epidemiology and Microbiology

Email: niiepit@rambler.ru
Rússia, Moscow, 123098

Z. Shevtsova

Research Institute of Experimental Pathology and Therapy

Autor responsável pela correspondência
Email: niiepit@rambler.ru
Rússia, Sukhumi, Abkhazia

A. Matua

Research Institute of Experimental Pathology and Therapy

Email: niiepit@rambler.ru
Rússia, Sukhumi, Abkhazia

I. Kondzariya

Research Institute of Experimental Pathology and Therapy

Email: niiepit@rambler.ru
Rússia, Sukhumi, Abkhazia

A. Amichba

Research Institute of Experimental Pathology and Therapy

Email: niiepit@rambler.ru
Rússia, Sukhumi, Abkhazia

D. Kubrava

Research Institute of Experimental Pathology and Therapy

Email: niiepit@rambler.ru
Rússia, Sukhumi, Abkhazia

Z. Mikvabia

Research Institute of Experimental Pathology and Therapy

Email: niiepit@rambler.ru
Rússia, Sukhumi, Abkhazia

Arquivos suplementares

Arquivos suplementares
Ação
1. JATS XML

Declaração de direitos autorais © Pleiades Publishing, Inc., 2016