Insertional inactivation of virulence operon in population of persistent Bordetella pertussis bacteria
- Autores: Karataev G.I.1, Sinyashina L.N.1, Medkova A.Y.1, Semin E.G.1, Shevtsova Z.V.2, Matua A.Z.2, Kondzariya I.G.2, Amichba A.A.2, Kubrava D.T.2, Mikvabia Z.Y.2
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Afiliações:
- Gamaleya Research Center for Epidemiology and Microbiology
- Research Institute of Experimental Pathology and Therapy
- Edição: Volume 52, Nº 4 (2016)
- Páginas: 370-377
- Seção: Genetics of Microorganisms
- URL: https://journals.rcsi.science/1022-7954/article/view/187675
- DOI: https://doi.org/10.1134/S102279541603008X
- ID: 187675
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Resumo
Avirulent B. pertussis bacteria containing IS elements in the bvgAS operon were detected during the study of whooping cough patients and bacilli carriers. The present work is devoted to the study of the accumulation dynamics and the mechanisms of generation of persistent forms of the B. pertussis bacteria in lower monkeys as the most adequate model for extrapolation of the experiment results to humans. By means of the real-time PCR method, it was established that the B. pertussis bacteria lived more than three months in the upper respiratory tract after a single intranasal monkey infection; the period was reduced to 14–28 days during repeated infection. An increase in the portion of B. pertussis Bvg mutants in the population to tens of percent from the total number of registered bacteria was registered. The experimental confirmation of the development and accumulation of avirulent B. pertussis Bvg mutants during the development of the infectious process was obtained. Further study of the composition of the B. pertussis persistent bacteria population at different stages of the disease will make it possible to formulate new approaches to the whooping cough diagnostics and prevention and creation of fundamentally new drugs.
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Sobre autores
G. Karataev
Gamaleya Research Center for Epidemiology and Microbiology
Autor responsável pela correspondência
Email: karataevgi@rambler.ru
Rússia, Moscow, 123098
L. Sinyashina
Gamaleya Research Center for Epidemiology and Microbiology
Email: niiepit@rambler.ru
Rússia, Moscow, 123098
A. Medkova
Gamaleya Research Center for Epidemiology and Microbiology
Email: niiepit@rambler.ru
Rússia, Moscow, 123098
E. Semin
Gamaleya Research Center for Epidemiology and Microbiology
Email: niiepit@rambler.ru
Rússia, Moscow, 123098
Z. Shevtsova
Research Institute of Experimental Pathology and Therapy
Autor responsável pela correspondência
Email: niiepit@rambler.ru
Rússia, Sukhumi, Abkhazia
A. Matua
Research Institute of Experimental Pathology and Therapy
Email: niiepit@rambler.ru
Rússia, Sukhumi, Abkhazia
I. Kondzariya
Research Institute of Experimental Pathology and Therapy
Email: niiepit@rambler.ru
Rússia, Sukhumi, Abkhazia
A. Amichba
Research Institute of Experimental Pathology and Therapy
Email: niiepit@rambler.ru
Rússia, Sukhumi, Abkhazia
D. Kubrava
Research Institute of Experimental Pathology and Therapy
Email: niiepit@rambler.ru
Rússia, Sukhumi, Abkhazia
Z. Mikvabia
Research Institute of Experimental Pathology and Therapy
Email: niiepit@rambler.ru
Rússia, Sukhumi, Abkhazia
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