Genomic Profiling of the Response of Aspergillus oryzae to the Treatment with Bis(2-Pyridine-1-Oxide) Diselenide
- Autores: Zalepkina S.1, Smirnov V.1, Borisov A.2, Matsulevich Z.2
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Afiliações:
- Department of Biochemistry and Biotechnology, Lobachevsky State University of Nizhny Novgorod
- Department of Industrial Safety, Ecology, and Chemistry, Nizhny Novgorod State Technical University
- Edição: Volume 55, Nº 3 (2019)
- Páginas: 301-308
- Seção: Genetics of Microorganisms
- URL: https://journals.rcsi.science/1022-7954/article/view/189270
- DOI: https://doi.org/10.1134/S1022795419030177
- ID: 189270
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Resumo
We implemented genome-wide expression profiling to identify the mechanisms of toxicity of an organoselenium compound bis(2-pyridine-1-oxide) diselenide to the fungus А. oryzae RIB40. We uncovered changes in the expression levels in 72 genes. In particular, we observed a downregulation in the levels of several copper ion transmembrane transporter genes. In turn, we found a significant upregulation in the genes encoding oxidoreductases. The latter results are supported by biochemical experiments that revealed an increase in oxidoreductase activity in response to bis(2-pyridine-1-oxide) diselenide treatment. The results of a large-scale microarray analysis of the А. oryzae RIB 40 were confirmed by real-time quantitative PCR.
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Sobre autores
S. Zalepkina
Department of Biochemistry and Biotechnology, Lobachevsky State University of Nizhny Novgorod
Email: biodeg@mail.ru
Rússia, Nizhny Novgorod, 603950
V. Smirnov
Department of Biochemistry and Biotechnology, Lobachevsky State University of Nizhny Novgorod
Autor responsável pela correspondência
Email: biodeg@mail.ru
Rússia, Nizhny Novgorod, 603950
A. Borisov
Department of Industrial Safety, Ecology, and Chemistry, Nizhny Novgorod State Technical University
Email: biodeg@mail.ru
Rússia, Nizhny Novgorod, 603950
Zh. Matsulevich
Department of Industrial Safety, Ecology, and Chemistry, Nizhny Novgorod State Technical University
Email: biodeg@mail.ru
Rússia, Nizhny Novgorod, 603950