Genetic Heterogeneity of a Diploid Grass Aegilops tauschii Revealed by Chromosome Banding Methods and Electrophoretic Analysis of the Seed Storage Proteins (Gliadins)
- Авторлар: Badaeva E.1,2, Fisenko A.3, Surzhikov S.2, Yankovskaya A.1, Chikida N.4, Zoshchuk S.2, Belousova M.4, Dragovich A.1
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Мекемелер:
- Vavilov Institute of General Genetics, Russian Academy of Sciences
- Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
- Tsitsin Main Moscow Botanical Garden, Russian Academy of Sciences
- Federal Research Center Vavilov All-Russian Institute of Plant Genetic Resources
- Шығарылым: Том 55, № 11 (2019)
- Беттер: 1315-1329
- Бөлім: Plant Genetics
- URL: https://journals.rcsi.science/1022-7954/article/view/189697
- DOI: https://doi.org/10.1134/S1022795419110024
- ID: 189697
Дәйексөз келтіру
Аннотация
Genetic diversity of diploid grass Ae. tauschii Coss (2n = 2x = 14, DD), the D-genome progenitor of common wheat, was assessed using fluorescence in situ hybridization (FISH) with eleven DNA probes representing satellite and microsatellite DNA sequences as well as the 45S and 5S rRNA gene families and by electrophoretic (EF) analysis of seed storage proteins (gliadins). A clear genetic differentiation of accessions into groups strangulata (Str) and tauschii (Tau) was observed. The groups differed in the presence of microsatellite repeats GAAn and ACTn and in the distribution of satellite DNA families, especially pAs1. On the basis of similarities of labeling patterns of DNA probes used in the study, we concluded that the Str group was phylogenetically closest to the D genome of common wheat. A comparison of spectra of gliadins revealed the highest similarity of Armenian and Azerbaijani accessions of Ae. tauschii to common wheat, which may indicate a contribution of Transcaucasian members of the Str group in the formation of the genetic pool of common wheat.
Негізгі сөздер
Авторлар туралы
E. Badaeva
Vavilov Institute of General Genetics, Russian Academy of Sciences; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Хат алмасуға жауапты Автор.
Email: katerinabadaeva@gmail.com
Ресей, Moscow, 119991; Moscow, 119991
A. Fisenko
Tsitsin Main Moscow Botanical Garden, Russian Academy of Sciences
Email: katerinabadaeva@gmail.com
Ресей, Moscow, 127276
S. Surzhikov
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Email: katerinabadaeva@gmail.com
Ресей, Moscow, 119991
A. Yankovskaya
Vavilov Institute of General Genetics, Russian Academy of Sciences
Email: katerinabadaeva@gmail.com
Ресей, Moscow, 119991
N. Chikida
Federal Research Center Vavilov All-Russian Institute of Plant Genetic Resources
Email: katerinabadaeva@gmail.com
Ресей, St. Petersburg, 190121
S. Zoshchuk
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Email: katerinabadaeva@gmail.com
Ресей, Moscow, 119991
M. Belousova
Federal Research Center Vavilov All-Russian Institute of Plant Genetic Resources
Email: katerinabadaeva@gmail.com
Ресей, St. Petersburg, 190121
A. Dragovich
Vavilov Institute of General Genetics, Russian Academy of Sciences
Email: katerinabadaeva@gmail.com
Ресей, Moscow, 119991