The New Role of СysB Transcription Factor in Cysteine Degradation and Production of Hydrogen Sulfide in E. coli
- 作者: Seregina T.A.1, Nagornykh M.O.1,2, Lobanov K.V.1, Shakulov R.S.3, Mironov A.S.1
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隶属关系:
- Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
- Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences
- State Research Institute of Genetics and Selection of Industrial Microorganisms
- 期: 卷 54, 编号 11 (2018)
- 页面: 1259-1265
- 栏目: Genetics of Microorganisms
- URL: https://journals.rcsi.science/1022-7954/article/view/188638
- DOI: https://doi.org/10.1134/S1022795418110145
- ID: 188638
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The paradoxical effect of deletion of the Escherichia coli genes cysK and cysM encoding cysteine synthase enzymes has been studied: such cysteine auxotrophs actively degrade the excess of cysteine transported from the medium to form H2S. We have shown that deletions of any of the known genes controlling the degradation of exogenous cysteine, including the genes aspC, mstA, cysK, cysM, tnaA, metC, and malY, as well as the newly discovered genes yciW, cyuA, cyuP, and cyuR, do not deprive the cysteine auxotrophs ΔcysK ΔcysM of the ability to degrade cysteine. Cysteine degradation in the ΔcysK ΔcysM mutant is positively regulated by the products of the cysB and cysE genes. It is significant that the ΔcysK ΔcysM mutant shows an increased transcription of the genes opposing the oxidative stress (sodA, catG, arcA, and cydD). We assume that oxidative stress in cells of the ΔcysK ΔcysM mutant is provoked by restriction of cysteine resynthesis, while cysB-dependent degradation of exogenous cysteine and generated H2S provide protection against oxidative stress.
作者简介
T. Seregina
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Email: alexmir_98@yahoo.com
俄罗斯联邦, Moscow, 119991
M. Nagornykh
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences; Institute of Biochemistry and Physiology of Microorganisms,Russian Academy of Sciences
Email: alexmir_98@yahoo.com
俄罗斯联邦, Moscow, 119991; Pushchino, Moscow oblast, 142290
K. Lobanov
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Email: alexmir_98@yahoo.com
俄罗斯联邦, Moscow, 119991
R. Shakulov
State Research Institute of Genetics and Selection of Industrial Microorganisms
Email: alexmir_98@yahoo.com
俄罗斯联邦, Moscow, 117545
A. Mironov
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
编辑信件的主要联系方式.
Email: alexmir_98@yahoo.com
俄罗斯联邦, Moscow, 119991
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