Localization of Chromosome Regions Controlling High Photosynthetic Potential in Russian Rice Cultivars
- Authors: Goncharova J.K.1, Gontcharov S.V.1, Chicharova E.E.1
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Affiliations:
- All-Russian Rice Research Institute
- Issue: Vol 54, No 7 (2018)
- Pages: 796-804
- Section: Plant Genetics
- URL: https://journals.rcsi.science/1022-7954/article/view/189054
- DOI: https://doi.org/10.1134/S1022795418070037
- ID: 189054
Cite item
Abstract
Breeding practice defined the main ways to increase crop yield: morphotype change, exploitation of heterosis, and increase in photosynthesis effectiveness. We identified donors and sources of high photosynthetic potential genetic system and polymorphic loci controlling photosynthetic efficiency in Russian rice cultivars. Thirty-two Russian rice cultivars were used as material. The content of chlorophyll a and b and carotenoids was measured at the beginning of tillering, heading, and flowering stages by a Genesys 8 spectrophotometer. Twenty plants of each cultivar in two replications were used for measurement. SSR markers were used. DNA was extracted by the STAB method. Polymerase chain reaction (PCR) and visualization of amplification products were performed under the International Rice Research Institute (IRRI) protocol. Donors of high contents of each pigment were found. A high level of polymorphism for the loci connected with photosynthesis effectiveness in Russian rice cultivars will allow us to create new cultivars with improved characters by hybridization. Six markers significantly divided cultivar groups with different photosynthetic rate, three of them (RM154, RM600, RM5508) were related to the content of carotenoids, two markers (RM347, RM240) were related to specific leaf weight (SLW) control, and two others (RM154, RM509) were related to the chlorophyll a content.
About the authors
J. K. Goncharova
All-Russian Rice Research Institute
Author for correspondence.
Email: serggontchar@mail.ru
Russian Federation, Krasnodar, 3350921
S. V. Gontcharov
All-Russian Rice Research Institute
Email: serggontchar@mail.ru
Russian Federation, Krasnodar, 3350921
E. E. Chicharova
All-Russian Rice Research Institute
Email: serggontchar@mail.ru
Russian Federation, Krasnodar, 3350921