Email this article Testing Safety of Genetically Modified Products of Rice: Case Study on Sprague Dawley Rats
- Authors: Shirdeli M.1,2, Orlov Y.L.3,4, Eslami G.1,5, Hajimohammadi B.1,2, Tabikhanova L.E.3, Ehrampoush M.H.1, Rezvani M.E.6, Fallahzadeh H.7, Zandi H.1,8, Hosseini S.1, Ahmadian S.1,5, Mortazavi S.1, Fallahi R.9, Asadi-Yousefabad S.10
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Affiliations:
- Research Center for Food Hygiene and Safety, Shahid Sadoughi University of Medical Sciences
- Department of Food Hygiene and Safety, School of Health, Shahid Sadoughi University of Medical Sciences
- Institute of Cytology and Genetics SB RAS
- Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation (Sechenov University)
- Department of Parasitology and Mycology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences
- Department of Physiology, School of Medicine, Shahid Sadoughi University of Medical Sciences
- Department of Biostatistics and Epidemiology, Daneshjoo Boulevard, Health School, Shahid Sadoughi University of Medical Sciences
- Department of Microbiology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences
- Animal Viral Diseases Research Department, Razi Vaccine and Serum Research Institute, Agricultural Research Education and Extension Organization (AREEO)
- Student Research Committee, Shahid Sadoughi University of Medical Sciences
- Issue: Vol 55, No 8 (2019)
- Pages: 962-968
- Section: Plant Genetics
- URL: https://journals.rcsi.science/1022-7954/article/view/189491
- DOI: https://doi.org/10.1134/S1022795419080131
- ID: 189491
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Abstract
Genetic engineering is considered as background for crop protection against pest damage by adding new genes inside the grains. Rice, like other cereals is included in gene engineering experiments. The questions about possible gene transfer related to food safety appear. It is important to find any additional genes or fragments in animal tissues after consumption of genetically modified (GM) food. Therefore, in this study, the remaining of CryIA(b) gene and P35 were assessed in the liver of rats fed with GM rice. This work presents an experimental study with the intervention of GM rice feeding by Sprague Dawley rats. Overall, 20 male and 20 female SD rats were fed by pellets made by GM rice in 50% of needed carbohydrate for 90 days. Then, sampling was done from rats liver. DNA extraction was done based on the protocol. The quality and quantity of the extracted DNA was done by agarose gel electrophoresis and spectrophotometry, respectively. Detection of GM genes residues, including CryIA(b), P35, and T35 was done by Polymerase Chain Reaction using specific primer pairs. The results were analyzed by agarose gel electrophoresis alongside with 50 bp DNA ladder. The results were compared with the ones in control groups with feeding by standard pellet of non-modified rice. All amplification tests were done in triplicates. Analysis of the amplification of P35, CryIA(b) and T35 showed no residues inside the liver tissue. The results showed no significant difference in the presence of transgenic gene of cryIA(b), T35, and P35 in the liver tissue between the control and experiment groups. Therefore, this study rejects the possibility of gene settle of GM rice gene residues in liver tissue of the animal model studied.
Keywords
About the authors
M. Shirdeli
Research Center for Food Hygiene and Safety, Shahid Sadoughi University of Medical Sciences; Department of Food Hygiene and Safety, School of Health, Shahid Sadoughi Universityof Medical Sciences
Email: b.hajimohammadi@gmail.com
Iran, Islamic Republic of, Yazd; Yazd
Y. L. Orlov
Institute of Cytology and Genetics SB RAS; Sechenov First Moscow State Medical University of the Ministry of Healthof the Russian Federation (Sechenov University)
Author for correspondence.
Email: orlov@bionet.nsc.ru
Russian Federation, Novosibirsk, 630090; Moscow, 119991
G. Eslami
Research Center for Food Hygiene and Safety, Shahid Sadoughi University of Medical Sciences; Department of Parasitology and Mycology, Faculty of Medicine, Shahid Sadoughi Universityof Medical Sciences
Author for correspondence.
Email: eslami_g2000@yahoo.com
Iran, Islamic Republic of, Yazd; Yazd
B. Hajimohammadi
Research Center for Food Hygiene and Safety, Shahid Sadoughi University of Medical Sciences; Department of Food Hygiene and Safety, School of Health, Shahid Sadoughi Universityof Medical Sciences
Author for correspondence.
Email: b.hajimohammadi@gmail.com
Iran, Islamic Republic of, Yazd; Yazd
L. E. Tabikhanova
Institute of Cytology and Genetics SB RAS
Email: b.hajimohammadi@gmail.com
Russian Federation, Novosibirsk, 630090
M. H. Ehrampoush
Research Center for Food Hygiene and Safety, Shahid Sadoughi University of Medical Sciences
Email: b.hajimohammadi@gmail.com
Iran, Islamic Republic of, Yazd
M. E. Rezvani
Department of Physiology, School of Medicine, Shahid Sadoughi University of Medical Sciences
Email: b.hajimohammadi@gmail.com
Iran, Islamic Republic of, Yazd
H. Fallahzadeh
Department of Biostatistics and Epidemiology, Daneshjoo Boulevard, Health School,Shahid Sadoughi University of Medical Sciences
Email: b.hajimohammadi@gmail.com
Iran, Islamic Republic of, Yazd
H. Zandi
Research Center for Food Hygiene and Safety, Shahid Sadoughi University of Medical Sciences; Department of Microbiology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences
Email: b.hajimohammadi@gmail.com
Iran, Islamic Republic of, Yazd; Yazd
S. Hosseini
Research Center for Food Hygiene and Safety, Shahid Sadoughi University of Medical Sciences
Email: b.hajimohammadi@gmail.com
Iran, Islamic Republic of, Yazd
S. Ahmadian
Research Center for Food Hygiene and Safety, Shahid Sadoughi University of Medical Sciences; Department of Parasitology and Mycology, Faculty of Medicine, Shahid Sadoughi Universityof Medical Sciences
Email: b.hajimohammadi@gmail.com
Iran, Islamic Republic of, Yazd; Yazd
S. Mortazavi
Research Center for Food Hygiene and Safety, Shahid Sadoughi University of Medical Sciences
Email: b.hajimohammadi@gmail.com
Iran, Islamic Republic of, Yazd
R. Fallahi
Animal Viral Diseases Research Department, Razi Vaccine and Serum Research Institute, AgriculturalResearch Education and Extension Organization (AREEO)
Email: b.hajimohammadi@gmail.com
Iran, Islamic Republic of, Karaj
S. Asadi-Yousefabad
Student Research Committee, Shahid Sadoughi University of Medical Sciences
Email: b.hajimohammadi@gmail.com
Iran, Islamic Republic of, Yazd
