Отправить статью по E-mail Establishing a Cultivable Cell Line of the Tick Dermacentor marginatus
- Авторы: Rimikhanov N.I.1, Epova E.Y.2, Belyakova A.V.2, Lebedeva A.A.3, Mutnykh E.S.3, Biryukova Y.K.4, Zylkova M.V.4, Shibaeva A.V.2, Trubnikova E.V.4, Karataeva D.A.5, Akbaev R.M.5, Tyno Y.Y.5, Laypanov B.K.5
-
Учреждения:
- Department of Veterinary Medicine, Moscow State University of Food Production
- Emanuel Institute of Biochemical Physics, Russian Academy of Sciences
- Vavilov Institute of General Genetics, Russian Academy of Sciences
- Research Laboratory Genetika, Kursk State University
- Department of Parasitology and Veterinary-Sanitary Examination, Skryabin Moscow State Academy of Veterinary Medicine and Biotechnology
- Выпуск: Том 55, № 8 (2019)
- Страницы: 933-938
- Раздел: General Genetics
- URL: https://journals.rcsi.science/1022-7954/article/view/189483
- DOI: https://doi.org/10.1134/S102279541908012X
- ID: 189483
Цитировать
Открытый доступ
Доступ предоставлен
Только для подписчиков
Аннотация
Owing to the changes in the general ecological situation in Russia, livestock losses from ovine anaplasmosis have rapidly increased in recent years. The development of a vaccine against this disease is therefore all the more relevant. A continuous culture of tick cells could be used as an appropriate substrate for producing biomass of the pathogen of ovine anaplasmosis, Anaplasma ovis, for the purpose of manufacturing a cell-cultural inactivated vaccine. Such a continuous cell line of the tick Dermacentor marginatus, the definitive reservoir host of A. ovis, has been obtained by cultivation of homogenized tick eggs on a modified Leibovitz medium L15. The established cell line DM-77 has an undifferentiated phenotype: the cells are rounded, with large nuclei and well-visible nucleoli. By the end of the study, the cell line was passaged eight times and did not express signs of growth rate decrease. The duplication time was 2.8 ± 0.3 days for cultivation in a medium with 10% fetal serum at 32°C.
Ключевые слова
Об авторах
N. Rimikhanov
Department of Veterinary Medicine, Moscow State University of Food Production
Email: biriukova-ula@mail.ru
Россия, Moscow, 125080
E. Epova
Emanuel Institute of Biochemical Physics, Russian Academy of Sciences
Email: biriukova-ula@mail.ru
Россия, Moscow, 119334
A. Belyakova
Emanuel Institute of Biochemical Physics, Russian Academy of Sciences
Email: biriukova-ula@mail.ru
Россия, Moscow, 119334
A. Lebedeva
Vavilov Institute of General Genetics, Russian Academy of Sciences
Email: biriukova-ula@mail.ru
Россия, Moscow, 11991
E. Mutnykh
Vavilov Institute of General Genetics, Russian Academy of Sciences
Email: biriukova-ula@mail.ru
Россия, Moscow, 11991
Yu. Biryukova
Research Laboratory Genetika, Kursk State University
Автор, ответственный за переписку.
Email: biriukova-ula@mail.ru
Россия, Kursk, 305000
M. Zylkova
Research Laboratory Genetika, Kursk State University
Email: biriukova-ula@mail.ru
Россия, Kursk, 305000
A. Shibaeva
Emanuel Institute of Biochemical Physics, Russian Academy of Sciences
Email: biriukova-ula@mail.ru
Россия, Moscow, 119334
E. Trubnikova
Research Laboratory Genetika, Kursk State University
Email: biriukova-ula@mail.ru
Россия, Kursk, 305000
D. Karataeva
Department of Parasitology and Veterinary-Sanitary Examination, Skryabin Moscow State Academyof Veterinary Medicine and Biotechnology
Email: biriukova-ula@mail.ru
Россия, Moscow, 109472
R. Akbaev
Department of Parasitology and Veterinary-Sanitary Examination, Skryabin Moscow State Academyof Veterinary Medicine and Biotechnology
Email: biriukova-ula@mail.ru
Россия, Moscow, 109472
Y. Tyno
Department of Parasitology and Veterinary-Sanitary Examination, Skryabin Moscow State Academyof Veterinary Medicine and Biotechnology
Email: biriukova-ula@mail.ru
Россия, Moscow, 109472
B. Laypanov
Department of Parasitology and Veterinary-Sanitary Examination, Skryabin Moscow State Academyof Veterinary Medicine and Biotechnology
Email: biriukova-ula@mail.ru
Россия, Moscow, 109472
Дополнительные файлы
