Compensation Ability between the Chromosomes of Homoeologous Group 7 of Triticum aestivum L. and Hordeum marinum ssp. gussoneanum Hudson (2n = 28) and Analysis of the Transmission Frequency of Alien 7H1Lmar Chromosome through Gametes in the Progeny of Wheat–Barley Substitution Lines

Abstract

On the basis of directed chromosome substitution, ditelosomic (DT) wheat–barley substitution lines (2n = 42 = 40 + 2t) with participation of wild tetraploid barley Hordeum marinum ssp. gussoneanum Hudson (2n = 28) were first obtained and analyzed. GISH analysis of the substitution lines showed that they carried a pair of telocentric barley chromosomes. With the help of ditelosomic analysis, the lines were identified as DT7H^IL^mar(7A) and DT7H^IL^mar(7B). On the basis of the compensation test and the study of meiotic stability of substitution lines, it was demonstrated that 7H^IL^mar chromosome of H. marinum was homoeologous to the group 7 chromosomes of common wheat. Analysis of the gamete transfer frequency in the progeny of reciprocal hybrids between F₁ 7A-7H^IL^mar, 7B-7H^IL^mar, 7D-7H^IL^mar and Saratovskaya 29 cultivar showed that the gamete with alien chromosome n = 20 + t successfully competed with euploid gamete n = 21 in both macrosporogenesis and microsporogenesis.