Molecular Analysis of Callipyge Gene Mutation (C.267A>G) in Kajli, Lohi and Thalli Sheep Breeds of Pakistan
- Authors: Shah A.1, Aziz A.2, Ghafoor A.2,3, Zahur A.B.2, Yousaf M.2, Ishaq R.2, Abass N.2, Suleman M.4,3
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Affiliations:
- School of Life Sciences and Chinese Academy of Sciences, Key Laboratory of Brain Function and Disease
- National Institute for Genomics and Advanced Biotechnology
- Key State Laboratory of Cellular Stress Biology, School of Life Sciences
- Centre for Biotechnology and Microbiology
- Issue: Vol 54, No 7 (2018)
- Pages: 848-852
- Section: Animal Genetics
- URL: https://journals.rcsi.science/1022-7954/article/view/189072
- DOI: https://doi.org/10.1134/S1022795418070141
- ID: 189072
Cite item
Abstract
The Callipyge (CLPG) mutation has been reported in postnatal muscular hypertrophy which specifically functions in the pelvic limbs and loin of ovine. In current study, for the first time we investigated CLPG mutation (C.267A>G) by using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) and sequencing in three Pakistani sheep breeds, named as Thalli, Kajli and Lohi. The results showed that Thalli breed has mutant homozygous (GG, 0.02%), heterozygous (AG, 0.10%) and normal homozygous (AA, 0.88%) forms of CLPG. The sequence analysis of the purified PCR product of the Thalli sheep represents these three types of genotypes. However Kajli and Lohi breeds were found normal for this genetic variation of CLPG. On the basis of current findings and physical examination of these breeds, it was concluded that CLPG Thalli breed can be a good source of mutton and subsequent breeding of the Callipyge phenotype will greatly overcome the meat shortage in Pakistan.
About the authors
A. Shah
School of Life Sciences and Chinese Academy of Sciences, Key Laboratory of Brain Function and Disease
Author for correspondence.
Email: shahbiotech3@yahoo.com
China, Hefei
A. Aziz
National Institute for Genomics and Advanced Biotechnology
Email: shahbiotech3@yahoo.com
Pakistan, Islamabad
A. Ghafoor
National Institute for Genomics and Advanced Biotechnology; Key State Laboratory of Cellular Stress Biology, School of Life Sciences
Email: shahbiotech3@yahoo.com
Pakistan, Islamabad; Xiamen
A. B. Zahur
National Institute for Genomics and Advanced Biotechnology
Email: shahbiotech3@yahoo.com
Pakistan, Islamabad
M. Yousaf
National Institute for Genomics and Advanced Biotechnology
Email: shahbiotech3@yahoo.com
Pakistan, Islamabad
R. Ishaq
National Institute for Genomics and Advanced Biotechnology
Email: shahbiotech3@yahoo.com
Pakistan, Islamabad
N. Abass
National Institute for Genomics and Advanced Biotechnology
Email: shahbiotech3@yahoo.com
Pakistan, Islamabad
M. Suleman
Centre for Biotechnology and Microbiology; Key State Laboratory of Cellular Stress Biology, School of Life Sciences
Email: shahbiotech3@yahoo.com
Pakistan, Swat, KPK; Xiamen