Activities Analysis and Polymorphisms Identification of GPIHBP1 Promoter Region in Porcine

Abstract

GPIHBP1 is expressed restrictively in capillary endothelial cells, but the transcriptional regulation mechanisms of GPIHBP1 in capillaries are still unknown. Here, we analyzed the promoter activities of porcine GPIHBP1 using dual luciferase reporter assays and detected single nucleotide polymorphisms (SNPs) in the promoter region. Activity analysis discovered that the sequence from–187 to +174 is a basal promoter region, the sequence from–187 to–582 is a positive regulation region, whereas the sequence from–582 to–1321 is a negative regulation region. 11 SNPs were found in the promoter region of porcine GPIHBP1, and the g.-796G>A SNP within the NF-l-binding site is associated with pig back fat thickness. The work performed here will serve as a valuable resource for discovering the expression regulation mechanism of GPIHBP1 and promoting further development of pig as a model organism for human cardiovascular disease research.