Comparison of Some Plant DNA Extraction Methods
- Authors: Scobeyeva V.A.1, Omelchenko D.O.2, Dyakov L.M.2, Konovalov A.S.3, Speranskaya A.S.1, Krinitsina A.A.1
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Affiliations:
- Department of Biological Evolution, Department of Higher Plants
- Medical Institute, Center for Biotechnological and Bioinformatics Studies in Medicine
- NextBio Ltd.
- Issue: Vol 54, No 5 (2018)
- Pages: 576-586
- Section: Methods
- URL: https://journals.rcsi.science/1022-7954/article/view/188986
- DOI: https://doi.org/10.1134/S1022795418050095
- ID: 188986
Cite item
Abstract
DNA isolation is a routine procedure when performed in laboratory environment, yet in the field it may still remain problematic. This is especially true of some crop species bred for useful metabolites that may also hinder DNA extraction. Here we compare the efficiency of DNA extraction protocols and commercial DNA isolation kits when used on samples from Helianthus and Allium. Since extraction of DNA is known to be compromised by co-extraction of PCR-inhibiting metabolites, the isolation of DNA was followed by PCR as a testing procedure for the isolation step. The MagnoPrime Fact and MagnoPrime Uni DNA isolation kits were better suited for field work due to faster processing times and smaller required amount of starting material (20 mg fresh/0.5 mg dry). In all cases the subsequent PCR managed to amplify the DNA fragments of interest well enough to be useful in further research.
Keywords
About the authors
V. A. Scobeyeva
Department of Biological Evolution, Department of Higher Plants
Email: omdeno@gmail.com
Russian Federation, Moscow, 119234
D. O. Omelchenko
Medical Institute, Center for Biotechnological and Bioinformatics Studies in Medicine
Author for correspondence.
Email: omdeno@gmail.com
Russian Federation, Moscow, 117198
L. M. Dyakov
Medical Institute, Center for Biotechnological and Bioinformatics Studies in Medicine
Email: omdeno@gmail.com
Russian Federation, Moscow, 117198
A. S. Konovalov
NextBio Ltd.
Email: omdeno@gmail.com
Russian Federation, Moscow, 111394
A. S. Speranskaya
Department of Biological Evolution, Department of Higher Plants
Email: omdeno@gmail.com
Russian Federation, Moscow, 119234
A. A. Krinitsina
Department of Biological Evolution, Department of Higher Plants
Email: omdeno@gmail.com
Russian Federation, Moscow, 119234