Polymorphism of PIT-1 and Prolactin Genes and Their Effects on Milk Yield in Holstein Frisian Dairy Cows Bred in Vietnam
- Authors: Thuy N.T.1, Thu N.T.1, Cuong N.H.1, Ty L.V.1, Nguyen T.T.2, Khoa D.V.3
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Affiliations:
- Institute of Biotechnology
- Vietnam National University of Agriculture
- College of Agriculture and Applied Biology
- Issue: Vol 54, No 3 (2018)
- Pages: 346-352
- Section: Animal Genetics
- URL: https://journals.rcsi.science/1022-7954/article/view/188886
- DOI: https://doi.org/10.1134/S1022795418030146
- ID: 188886
Cite item
Abstract
The aim of this study is to investigate the polymorphism of the Pituitary transcription factor (PIT-1) and Prolactin (PRL) genes in Holstein Friesian cows (HF) bred in Vietnam and their effects on milk yields. For these purposes, 125 HF dairy cows of milking age from Da Lat Milk Farm located in Lam Dong, Vietnam were sampled. Phenotypic data of milk yields (calculated on 305-day of milking cycle; kg/day) of each individual milking cow were collected. The PIT-1.E2, PIT-1.E6, PRL.E3 and PRL.E4 genotypes were analyzed using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method with restriction enzymes TaqI, HinfI, RsaI, respectively. The significant effects of PIT-1.E6, PRL.E3 and PRL.E4 polymorphisms on milk yields were not observed in HF dairy cows (P > 0.05). However, results revealed statistically significant associations between PIT-1.E2 polymorphism and milk yields of HF dairy cows; individuals carrying the AA genotype yielded more milk compared to others. Our study suggests the potential use of PIT-1.E2 SNP in marker assisted selection (MAS) for improved milk yields of HF dairy cows bred in Vietnam.
About the authors
N. T. D. Thuy
Institute of Biotechnology
Author for correspondence.
Email: ntdthuy@ibt.ac.vn
Viet Nam, Hanoi
N. T. Thu
Institute of Biotechnology
Email: ntdthuy@ibt.ac.vn
Viet Nam, Hanoi
N. H. Cuong
Institute of Biotechnology
Email: ntdthuy@ibt.ac.vn
Viet Nam, Hanoi
L. V. Ty
Institute of Biotechnology
Email: ntdthuy@ibt.ac.vn
Viet Nam, Hanoi
T. T. B. Nguyen
Vietnam National University of Agriculture
Email: ntdthuy@ibt.ac.vn
Viet Nam, Hanoi
D. V. A. Khoa
College of Agriculture and Applied Biology
Email: ntdthuy@ibt.ac.vn
Viet Nam, CanTho