Genetic Structure of the Hyrcanian Wood Frog, Rana pseudodalmatina (Amphibia: Ranidae) Using mtDNA Gene Sequences
- Authors: Najibzadeh M.1, Gharzi A.1, Rastegar-Pouyani N.1, Rastegar-Pouyani E.2, Pesarakloo A.3
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Affiliations:
- Department of Biology, Faculty of Science, Razi University
- Department of Biology, Faculty of Science, Hakim Sabzevari University
- Department of Biology, Faculty of Science, Arak University
- Issue: Vol 54, No 10 (2018)
- Pages: 1221-1228
- Section: Animal Genetics
- URL: https://journals.rcsi.science/1022-7954/article/view/188621
- DOI: https://doi.org/10.1134/S1022795418100095
- ID: 188621
Cite item
Abstract
Population structuring of a species provide basic information for biological conservation. We investigated the genetic structure of seven populations of Rana pseudodalmatina, an endemic species of the Ranidae inhabiting the Hyrcanian forests in northern Iran, based on the genetic variation of two partial mitochondrial DNA sequences (16S rRNA and cytochrome b genes). Molecular genetic analyses revealed remarkable variation among populations of R. pseudodalmatina. The phylogenetic trees clearly indicated two distinct haplogroups, which largely corresponded to their geographic locations. A strong population structure was found (ΦCT = 0.559, P = 0.027) with high haplotype diversity (Hd = 0.88) and low nucleotide diversity (π = 0.0041). Analysis of molecular variance (AMOVA) indicated that most of the observed genetic variation (55.92%) occurred between the two haplogroups. Also, Mantel tests revealed a significant correlation between geographic and genetic distances (R2 = 0.33, P = 0.005). Finally, the star-like topology of haplotype network and also neutrality tests provide strong evidence for population expansion in two haplogroups. All the findings of the present study, suggest a strong evidence for past expansion of isolated populations of R. pseudodalmatina, which their isolation could be largely attributed to rising level of the Caspian Sea during last glacial periods in the Pleistocene.
About the authors
M. Najibzadeh
Department of Biology, Faculty of Science, Razi University
Email: ahgharzi@yahoo.com
Iran, Islamic Republic of, Kermanshah, 6714967346
A. Gharzi
Department of Biology, Faculty of Science, Razi University
Author for correspondence.
Email: ahgharzi@yahoo.com
Iran, Islamic Republic of, Kermanshah, 6714967346
N. Rastegar-Pouyani
Department of Biology, Faculty of Science, Razi University
Email: ahgharzi@yahoo.com
Iran, Islamic Republic of, Kermanshah, 6714967346
E. Rastegar-Pouyani
Department of Biology, Faculty of Science, Hakim Sabzevari University
Email: ahgharzi@yahoo.com
Iran, Islamic Republic of, Sabzevar
A. Pesarakloo
Department of Biology, Faculty of Science, Arak University
Email: ahgharzi@yahoo.com
Iran, Islamic Republic of, Arak, 3815688349