Molecular genetic analysis of collection of transgenic tobacco plants with buckwheat serine proteases inhibitor gene during long-term subculture
- Authors: Khadeeva N.V.1, Yakovleva E.Y.1, Sydoruk K.V.2, Korostyleva T.V.1, Istomina E.A.1, Dunaevsky Y.E.3, Odintsova T.I.1, Bogush V.G.2, Belozersky M.A.3
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Affiliations:
- Vavilov Institute of General Genetics
- State Scientific Research Institute of Genetics and Selection of Industrial Microorganisms
- Belozersky Institute of Physicochemical Biology
- Issue: Vol 53, No 11 (2017)
- Pages: 1200-1210
- Section: Plant Genetics
- URL: https://journals.rcsi.science/1022-7954/article/view/188511
- DOI: https://doi.org/10.1134/S1022795417110047
- ID: 188511
Cite item
Abstract
In this paper, the results of long-term screening of independently derived transgenic tobacco plants carrying the synthetic BWI-1a gene of serine proteases inhibitor from buckwheat are presented. For several years periodic spot checks of persistence and expression of the heterologous protective genes in vegetatively cloned collections and seeds of transgenic plants were conducted. The persistence of expression of the target gene after ten years of passage of plants in aseptic culture without selective pressure in their seed progeny for at least three generations and derived callus was shown. Extracts of tissues of all the variants of transgenic plants inhibited the growth of phytopathogenic bacteria and the germination of spores of fungi. The degree of the suppression of a pathogen in this case was hardly reduced. In the second seed generation, the number of defective seeds increased and there was a sharp decline of germination ability of the seeds even in nonselective conditions.
About the authors
N. V. Khadeeva
Vavilov Institute of General Genetics
Author for correspondence.
Email: khadeeva@vigg.ru
Russian Federation, Moscow, 119991
E. Yu. Yakovleva
Vavilov Institute of General Genetics
Email: khadeeva@vigg.ru
Russian Federation, Moscow, 119991
K. V. Sydoruk
State Scientific Research Institute of Genetics and Selection of Industrial Microorganisms
Email: khadeeva@vigg.ru
Russian Federation, Moscow, 117545
T. V. Korostyleva
Vavilov Institute of General Genetics
Email: khadeeva@vigg.ru
Russian Federation, Moscow, 119991
E. A. Istomina
Vavilov Institute of General Genetics
Email: khadeeva@vigg.ru
Russian Federation, Moscow, 119991
Ya. E. Dunaevsky
Belozersky Institute of Physicochemical Biology
Email: khadeeva@vigg.ru
Russian Federation, Moscow, 119992
T. I. Odintsova
Vavilov Institute of General Genetics
Email: khadeeva@vigg.ru
Russian Federation, Moscow, 119991
V. G. Bogush
State Scientific Research Institute of Genetics and Selection of Industrial Microorganisms
Email: khadeeva@vigg.ru
Russian Federation, Moscow, 117545
M. A. Belozersky
Belozersky Institute of Physicochemical Biology
Email: khadeeva@vigg.ru
Russian Federation, Moscow, 119992