Comparative analysis of  Agropyron intermedium  (Host) Beauv 6Ag i  and 6Ag i 2 chromosomes in bread wheat cultivars and lines with wheat–wheatgrass substitutions

S. N. Sibikeev; E. D. Badaeva; E. I. Gultyaeva; A. E. Druzhin; A. A. Shishkina; A. Yu. Dragovich; P. Yu. Kroupin; G. I. Karlov; Thi Mai Khuat; M. G. Divashuk

doi:10.1134/S1022795417030115

Comparative analysis of Agropyron intermedium (Host) Beauv 6Agⁱ and 6Agⁱ2 chromosomes in bread wheat cultivars and lines with wheat–wheatgrass substitutions

Authors: Sibikeev S.N.¹, Badaeva E.D.², Gultyaeva E.I.³, Druzhin A.E.¹, Shishkina A.A.², Dragovich A.Y.², Kroupin P.Y.⁴, Karlov G.I.⁴, Khuat T.M.⁴, Divashuk M.G.⁴
Affiliations:
1. Agricultural Research Institute for Southeast Regions
2. Vavilov Institute of General Genetics
3. All-Russian Research Institute of Plant Protection
4. Center for Molecular Biotechnology
Issue: Vol 53, No 3 (2017)
Pages: 314-324
Section: Plant Genetics
URL: https://journals.rcsi.science/1022-7954/article/view/188156
DOI: https://doi.org/10.1134/S1022795417030115
ID: 188156

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Abstract

A comparative study of wheat–wheatgrass substituted cultivars and lines resistant to leaf rust developed by the Agricultural Research Institute for Southeast Regions (Multi 6R, Belyanka, Favorit, Voevoda, Lebedushka) and Samara Agricultural Research Institute (Tulaikovskaya 5, Tulaikovskaya 10, Tulaikovskaya 100, Tulaikovskaya Zolotistaya) breeding was conducted. A complex analysis using molecular cytogenetic (C-differential banding, fluorescent (FISH) and genomic (GISH) in situ hybridization), molecular (PLUG markers), and biochemical (electrophoretic analysis of gliadins) markers demonstrated that they have a substitition of wheat chromosome 6D by the chromosomes 6Agⁱ and 6Agⁱ2 belonging to the J(=E) Agropyron intermedium (Host) Beauv (=Thinopyrum intermedium (Host) Barkworth & D.R. Dewey) subgenome. In spite of the fact that the chromosomes 6Agⁱ and 6Agⁱ2 differ in the C-banding pattern and demonstrated minor differences in the blocks of gliadin components, they had the identical pattern of pSc119.2 and pAs1 probe distribution and conjugated between themselves with insignificant disturbance. Thus, it was demonstrated that 6Agi and 6Agⁱ2 are homologous chromosomes; however, the question about allelism of their leaf rust resistance genes between themselves requires special studies. Nevertheless, using STS and SCAR markers and taking into account the type of reaction to Puccinia triticina, their non-allelism to the Lr9, Lr19, Lr24, Lr29, Lr38, and Lr47 genes was established. It was revealed that the 6Agⁱ and 6Agⁱ2 chromosomes have a different level of transmission in hybrid F₂ populations depending on the hybrid combination gene background.

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wheat–wheatgrass substituted cultivars, 6Agⁱ and 6Agⁱ2 chromosomes

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Comparative analysis of Agropyron intermedium (Host) Beauv 6Agi and 6Agi2 chromosomes in bread wheat cultivars and lines with wheat–wheatgrass substitutions

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Comparative analysis of Agropyron intermedium (Host) Beauv 6Agⁱ and 6Agⁱ2 chromosomes in bread wheat cultivars and lines with wheat–wheatgrass substitutions