Escherichia coli ydiO and ydiQRST genes encode components of acyl-CoA dehydrogenase complex of anaerobic fatty acid β-oxidation pathway
- Authors: Gulevich A.Y.1, Skorokhodova A.Y.1, Debabov V.G.1
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Affiliations:
- State Research Institute of Genetics and Selection of Industrial Microorganisms
- Issue: Vol 52, No 10 (2016)
- Pages: 1101-1104
- Section: Short Communications
- URL: https://journals.rcsi.science/1022-7954/article/view/187949
- DOI: https://doi.org/10.1134/S1022795416100021
- ID: 187949
Cite item
Abstract
Escherichia coli open reading frames ydiO and ydiQRST were identified as genes encoding components of the acyl-CoA dehydrogenase complex of anaerobic fatty acid β-oxidation. Individual or concomitant inactivation of fadE gene, encoding known aerobic acyl-CoA dehydrogenase, and ydiO and/or ydiQRST genes did not affect cellular growth on glucose as a sole carbon source. Aerobic growth on sodium oleate was observed only for the cells with intact fadE gene. With an alternative electron acceptor, the cells possessing intact fadE gene demonstrated anaerobic growth on sodium oleate irrespective of the presence or absence of ydiO and ydiQRST genes. For the fadE-deficient mutants, anaerobic growth on sodium oleate was observed only for cells with intact ydiO and ydiQRST genes, while the fadE/ydiO and fadE/ydiQRST mutants failed to grow under the similar conditions.
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About the authors
A. Yu. Gulevich
State Research Institute of Genetics and Selection of Industrial Microorganisms
Author for correspondence.
Email: gulevich@genetika.ru
Russian Federation, Moscow, 117545
A. Yu. Skorokhodova
State Research Institute of Genetics and Selection of Industrial Microorganisms
Email: gulevich@genetika.ru
Russian Federation, Moscow, 117545
V. G. Debabov
State Research Institute of Genetics and Selection of Industrial Microorganisms
Email: gulevich@genetika.ru
Russian Federation, Moscow, 117545