Lactobacillus fermentum 90 TC-4 taxonomic status confirmation using whole genome sequencing and MALDI TOF mass spectrum
- Authors: Belova I.V.1, Tochilina A.G.1, Solovyeva I.V.1, Efimov E.I.1, Gorlova I.S.2, Ivanova T.P.1, Zhirnov V.A.1
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Affiliations:
- Blokhina Nizhny Novgorod Research Institute of Epidemiology and Microbiology
- MICROGEN
- Issue: Vol 52, No 9 (2016)
- Pages: 907-913
- Section: Genetics of Microorganisms
- URL: https://journals.rcsi.science/1022-7954/article/view/187867
- DOI: https://doi.org/10.1134/S1022795416090039
- ID: 187867
Cite item
Abstract
With the use of whole genome sequencing, the taxonomic status of Lactobacillus fermentum 90 TC-4 strain from Russian collections were studied. Complex analysis of phenotypical and genetic properties was conducted using phenotypic and molecular genetic methods. The main characteristics of the genome and biochemical activity profile of the strain were determined. A comparative analysis of the mass spectrum of ribosomal proteins of the strain, its biochemical properties, a fragment of 16S rRNA gene sequencing, and the entire genome revealed that the present strain belongs to the species L. fermentum, confirming its taxonomic status in accordance with modern taxonomy.
About the authors
I. V. Belova
Blokhina Nizhny Novgorod Research Institute of Epidemiology and Microbiology
Author for correspondence.
Email: lab-lb@yandex.ru
Russian Federation, Nizhny Novgorod, 603950
A. G. Tochilina
Blokhina Nizhny Novgorod Research Institute of Epidemiology and Microbiology
Email: lab-lb@yandex.ru
Russian Federation, Nizhny Novgorod, 603950
I. V. Solovyeva
Blokhina Nizhny Novgorod Research Institute of Epidemiology and Microbiology
Email: lab-lb@yandex.ru
Russian Federation, Nizhny Novgorod, 603950
E. I. Efimov
Blokhina Nizhny Novgorod Research Institute of Epidemiology and Microbiology
Email: lab-lb@yandex.ru
Russian Federation, Nizhny Novgorod, 603950
I. S. Gorlova
MICROGEN
Email: lab-lb@yandex.ru
Russian Federation, Nizhny Novgorod, 603950
T. P. Ivanova
Blokhina Nizhny Novgorod Research Institute of Epidemiology and Microbiology
Email: lab-lb@yandex.ru
Russian Federation, Nizhny Novgorod, 603950
V. A. Zhirnov
Blokhina Nizhny Novgorod Research Institute of Epidemiology and Microbiology
Email: lab-lb@yandex.ru
Russian Federation, Nizhny Novgorod, 603950